Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13578 | 5' | -57.1 | NC_003444.1 | + | 10120 | 0.66 | 0.496613 |
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Target: 5'- -uUCuGGAAAucaGCCGUugUACCGGGUa -3' miRNA: 3'- cuAGuCCUUUug-CGGCG--GUGGCCCGa -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 6545 | 0.66 | 0.475669 |
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Target: 5'- uGGUgAGGGAAAcCGCCGCauCACCGGu-- -3' miRNA: 3'- -CUAgUCCUUUU-GCGGCG--GUGGCCcga -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 14907 | 0.67 | 0.435165 |
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Target: 5'- uGAUCuGGAuaucCGCCG-CACCGGGa- -3' miRNA: 3'- -CUAGuCCUuuu-GCGGCgGUGGCCCga -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 20939 | 0.67 | 0.435165 |
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Target: 5'- --cCuGGAAAGCGgCGCUGagaCGGGCUg -3' miRNA: 3'- cuaGuCCUUUUGCgGCGGUg--GCCCGA- -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 14758 | 0.67 | 0.406132 |
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Target: 5'- cAUCAGGuucAGC-CCGCCGCCaGGUg -3' miRNA: 3'- cUAGUCCuu-UUGcGGCGGUGGcCCGa -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 25609 | 0.69 | 0.326728 |
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Target: 5'- --aCAGGcAGGACGCCcacgaaguaGCCGCCugGGGCa -3' miRNA: 3'- cuaGUCC-UUUUGCGG---------CGGUGG--CCCGa -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 8060 | 0.7 | 0.273342 |
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Target: 5'- cGGUgGGGGcgAAGUGCUGCCAgCGGGCg -3' miRNA: 3'- -CUAgUCCU--UUUGCGGCGGUgGCCCGa -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 32490 | 0.72 | 0.209506 |
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Target: 5'- ------cAAAACGCCGCCGCUGGGUUa -3' miRNA: 3'- cuaguccUUUUGCGGCGGUGGCCCGA- -5' |
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| 13578 | 5' | -57.1 | NC_003444.1 | + | 9944 | 1.08 | 0.000416 |
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Target: 5'- cGAUCAGGAAAACGCCGCCACCGGGCUg -3' miRNA: 3'- -CUAGUCCUUUUGCGGCGGUGGCCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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