miRNA: 3'- -GGAGCCGuCGC--UAGa-CG-UUGGCGCGC- -5'
miRNA: 3'- -GGaGCCGUCGC-UAgACguUGGCgCGC- -5'
miRNA: 3'- -GGAGC-CGUCG--CUagaCGUUGGCGCGc -5'
miRNA: 3'- -GGAGCcGUCGCUa---GACGU---------------UGGCGCGc -5'
miRNA: 3'- -GGAGCCGUCGCUAGACGUUGGCGCGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13579 | 3' | -57.8 | NC_003444.1 | + | 7975 | 0.68 | 0.348107 |
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Target: 5'- uCUUCGGCuGUGuuGUCacGCuGACCGCGCGu -3' miRNA: 3'- -GGAGCCGuCGC--UAGa-CG-UUGGCGCGC- -5' |
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| 13579 | 3' | -57.8 | NC_003444.1 | + | 6894 | 0.7 | 0.285594 |
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Target: 5'- uCC-CGGCGGCGuAUgUGguACCGgGUGa -3' miRNA: 3'- -GGaGCCGUCGC-UAgACguUGGCgCGC- -5' |
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| 13579 | 3' | -57.8 | NC_003444.1 | + | 1615 | 0.7 | 0.278434 |
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Target: 5'- cCCUUGaGCAGUucGAgggGCAGCCGUGCa -3' miRNA: 3'- -GGAGC-CGUCG--CUagaCGUUGGCGCGc -5' |
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| 13579 | 3' | -57.8 | NC_003444.1 | + | 8644 | 0.74 | 0.15661 |
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Target: 5'- aCCUCGaCAGCGAgacgCUGCAuaccagugcguauguACUGCGCa -3' miRNA: 3'- -GGAGCcGUCGCUa---GACGU---------------UGGCGCGc -5' |
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| 13579 | 3' | -57.8 | NC_003444.1 | + | 12391 | 1.11 | 0.00026 |
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Target: 5'- gCCUCGGCAGCGAUCUGCAACCGCGCGg -3' miRNA: 3'- -GGAGCCGUCGCUAGACGUUGGCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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