miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1358 5' -57.7 NC_001335.1 + 21441 0.66 0.557043
Target:  5'- gCUUCGCUGGccccGGAagGGCUGCUGAa--ACu -3'
miRNA:   3'- -GAGGCGACC----UCU--UCGACGACUgggUG- -5'
1358 5' -57.7 NC_001335.1 + 15073 0.66 0.524935
Target:  5'- -gCCGCUGcuGAAGCUGggccagaaGGCCCGCg -3'
miRNA:   3'- gaGGCGACcuCUUCGACga------CUGGGUG- -5'
1358 5' -57.7 NC_001335.1 + 25551 0.66 0.514386
Target:  5'- gCUCCGCUGGucAGAGGgUGUUuucGCCCu- -3'
miRNA:   3'- -GAGGCGACC--UCUUCgACGAc--UGGGug -5'
1358 5' -57.7 NC_001335.1 + 19968 0.66 0.503924
Target:  5'- aUuuGCUGGGuuGAAGCU-CgGACCCAUc -3'
miRNA:   3'- gAggCGACCU--CUUCGAcGaCUGGGUG- -5'
1358 5' -57.7 NC_001335.1 + 28641 0.67 0.457082
Target:  5'- -cCCGCaggcgacgaGGAGAAGCUGCUGaagggucaguugaacGCCCuCa -3'
miRNA:   3'- gaGGCGa--------CCUCUUCGACGAC---------------UGGGuG- -5'
1358 5' -57.7 NC_001335.1 + 16192 0.67 0.443292
Target:  5'- gCUCCGCUGGucGGcaucguggccGGuCUGCUGGCCgGu -3'
miRNA:   3'- -GAGGCGACCu-CU----------UC-GACGACUGGgUg -5'
1358 5' -57.7 NC_001335.1 + 42258 0.68 0.433587
Target:  5'- --gCGCUGGuGAucgAGCUGUacGGCCCGCc -3'
miRNA:   3'- gagGCGACCuCU---UCGACGa-CUGGGUG- -5'
1358 5' -57.7 NC_001335.1 + 26635 0.68 0.390593
Target:  5'- aUCCGCgUGGAGcgcgagucggucgagGAGCUGaugcGACCCAUc -3'
miRNA:   3'- gAGGCG-ACCUC---------------UUCGACga--CUGGGUG- -5'
1358 5' -57.7 NC_001335.1 + 34796 0.71 0.275124
Target:  5'- -aCUGCUGGGu-AGCUgGCUGGCCCGa -3'
miRNA:   3'- gaGGCGACCUcuUCGA-CGACUGGGUg -5'
1358 5' -57.7 NC_001335.1 + 17527 1.09 0.000456
Target:  5'- gCUCCGCUGGAGAAGCUGCUGACCCACu -3'
miRNA:   3'- -GAGGCGACCUCUUCGACGACUGGGUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.