Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13580 | 3' | -51.7 | NC_003444.1 | + | 14630 | 0.66 | 0.815742 |
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Target: 5'- -aCGGUaaacgcaCCUGCGCCAGCGcaccGCUggaUCCg -3' miRNA: 3'- ggGCUAac-----GGACGCGGUUGU----UGA---AGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 16301 | 0.66 | 0.785491 |
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Target: 5'- aCCCuuc-GCCaGCGUCAGCAACggCUg -3' miRNA: 3'- -GGGcuaaCGGaCGCGGUUGUUGaaGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 16117 | 0.67 | 0.763356 |
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Target: 5'- gCCCGGU--CCUGCauggucaGCCAGCGA--UCCg -3' miRNA: 3'- -GGGCUAacGGACG-------CGGUUGUUgaAGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 29842 | 0.67 | 0.742762 |
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Target: 5'- uUCUGAccaGUCaUGCGCCAGCGcguuguCUUCCg -3' miRNA: 3'- -GGGCUaa-CGG-ACGCGGUUGUu-----GAAGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 8474 | 0.67 | 0.736163 |
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Target: 5'- gCUGGUggguaugcUGCCUGCGCCGaaggggaaacgguucACGAUgaCCg -3' miRNA: 3'- gGGCUA--------ACGGACGCGGU---------------UGUUGaaGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 7776 | 0.7 | 0.571674 |
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Target: 5'- -gCGAUaaGCCUcgaccauacGCGCCAGCuGACUUCCc -3' miRNA: 3'- ggGCUAa-CGGA---------CGCGGUUG-UUGAAGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 2686 | 0.7 | 0.571674 |
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Target: 5'- cCCCuugUGCCUGCGCauaacaugCAACGGCaUCUg -3' miRNA: 3'- -GGGcuaACGGACGCG--------GUUGUUGaAGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 7844 | 0.76 | 0.259861 |
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Target: 5'- aCCG-UUGCCgccGCGCCugUGGCUUCCg -3' miRNA: 3'- gGGCuAACGGa--CGCGGuuGUUGAAGG- -5' |
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| 13580 | 3' | -51.7 | NC_003444.1 | + | 12525 | 1.16 | 0.000498 |
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Target: 5'- aCCCGAUUGCCUGCGCCAACAACUUCCg -3' miRNA: 3'- -GGGCUAACGGACGCGGUUGUUGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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