Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13581 | 5' | -60.7 | NC_003444.1 | + | 34300 | 0.67 | 0.270624 |
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Target: 5'- ---aGUGGCuggaucacugucuGGCCCAGuuCUGGCGGGUGu -3' miRNA: 3'- gcgaCACCG-------------UCGGGUU--GGUCGCCCAC- -5' |
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| 13581 | 5' | -60.7 | NC_003444.1 | + | 14795 | 0.67 | 0.25776 |
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Target: 5'- gGCUGUGGCugcuucAGCgcagcaaacugaCCAAUCAGCuGGUGc -3' miRNA: 3'- gCGACACCG------UCG------------GGUUGGUCGcCCAC- -5' |
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| 13581 | 5' | -60.7 | NC_003444.1 | + | 15262 | 0.67 | 0.25776 |
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Target: 5'- cCGCgcaaGGgGGCCaCGgcuGCCAGCGGGUa -3' miRNA: 3'- -GCGaca-CCgUCGG-GU---UGGUCGCCCAc -5' |
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| 13581 | 5' | -60.7 | NC_003444.1 | + | 8398 | 0.68 | 0.21465 |
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Target: 5'- aGCUGgcGGCAagccguaccGCCCGugCAGCGGuGUu -3' miRNA: 3'- gCGACa-CCGU---------CGGGUugGUCGCC-CAc -5' |
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| 13581 | 5' | -60.7 | NC_003444.1 | + | 24958 | 0.7 | 0.168444 |
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Target: 5'- gCGUUGUGGUgaaugcGGCUCAGCgCAcGCGGGUu -3' miRNA: 3'- -GCGACACCG------UCGGGUUG-GU-CGCCCAc -5' |
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| 13581 | 5' | -60.7 | NC_003444.1 | + | 13846 | 1.09 | 0.00016 |
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Target: 5'- aCGCUGUGGCAGCCCAACCAGCGGGUGa -3' miRNA: 3'- -GCGACACCGUCGGGUUGGUCGCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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