Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13582 | 5' | -53.6 | NC_003444.1 | + | 8073 | 0.66 | 0.723868 |
Target: 5'- gUGCUGCCAgCGGgc-GUaCAGauuGCCGUGGc -3' miRNA: 3'- -ACGGCGGU-GCUuuaCA-GUC---UGGCACC- -5' |
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13582 | 5' | -53.6 | NC_003444.1 | + | 14287 | 0.66 | 0.6905 |
Target: 5'- gGuuGUUACGG--UGUCGGACCGg-- -3' miRNA: 3'- aCggCGGUGCUuuACAGUCUGGCacc -5' |
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13582 | 5' | -53.6 | NC_003444.1 | + | 29958 | 0.68 | 0.599736 |
Target: 5'- aGCUGCCaccaGCGAAGUGUgGuGGCCGg-- -3' miRNA: 3'- aCGGCGG----UGCUUUACAgU-CUGGCacc -5' |
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13582 | 5' | -53.6 | NC_003444.1 | + | 14130 | 1.11 | 0.000656 |
Target: 5'- gUGCCGCCACGAAAUGUCAGACCGUGGa -3' miRNA: 3'- -ACGGCGGUGCUUUACAGUCUGGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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