Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13583 | 5' | -55.6 | NC_003444.1 | + | 7383 | 0.66 | 0.607903 |
Target: 5'- cCUGACCC-UGcCCGcGGUGAccuUUUgCCCg -3' miRNA: 3'- -GACUGGGcAC-GGCaCCACU---AAA-GGGa -5' |
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13583 | 5' | -55.6 | NC_003444.1 | + | 13714 | 0.67 | 0.497721 |
Target: 5'- -gGGCCCGcagcGCgGUGGUGGUggCCUc -3' miRNA: 3'- gaCUGGGCa---CGgCACCACUAaaGGGa -5' |
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13583 | 5' | -55.6 | NC_003444.1 | + | 1330 | 0.74 | 0.198246 |
Target: 5'- uUGuACCCG-GCgGUGGUGAUggCCCa -3' miRNA: 3'- gAC-UGGGCaCGgCACCACUAaaGGGa -5' |
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13583 | 5' | -55.6 | NC_003444.1 | + | 14667 | 1.08 | 0.000644 |
Target: 5'- gCUGACCCGUGCCGUGGUGAUUUCCCUg -3' miRNA: 3'- -GACUGGGCACGGCACCACUAAAGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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