Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13588 | 5' | -58.6 | NC_003444.1 | + | 8575 | 0.66 | 0.367044 |
Target: 5'- aGCAGGGUCUGcUGCUcGGUcauCGUGaaCCGUu -3' miRNA: 3'- aCGUCUCAGAC-ACGA-CCG---GCAC--GGUA- -5' |
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13588 | 5' | -58.6 | NC_003444.1 | + | 28372 | 0.68 | 0.277943 |
Target: 5'- -uCAGGGUgaGUGCUGGCCcaGCUAUc -3' miRNA: 3'- acGUCUCAgaCACGACCGGcaCGGUA- -5' |
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13588 | 5' | -58.6 | NC_003444.1 | + | 5101 | 0.74 | 0.104631 |
Target: 5'- cGCGGcAGUCUGUGUUGuGCCGgucacugaugcUGCCAg -3' miRNA: 3'- aCGUC-UCAGACACGAC-CGGC-----------ACGGUa -5' |
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13588 | 5' | -58.6 | NC_003444.1 | + | 36921 | 0.97 | 0.001558 |
Target: 5'- cUGCAG-GUCUGUGCUGGCCGUGCCAUa -3' miRNA: 3'- -ACGUCuCAGACACGACCGGCACGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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