Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1359 | 5' | -57 | NC_001335.1 | + | 23093 | 0.66 | 0.602592 |
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Target: 5'- ---cGGCCccGUGCugGCCacaUGGUCGg -3' miRNA: 3'- gacaCCGGucCAUGugUGGg--ACCAGU- -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 34281 | 0.66 | 0.580702 |
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Target: 5'- uUGUGGCCGgcGGUGUGCGCC--GGUCGg -3' miRNA: 3'- gACACCGGU--CCAUGUGUGGgaCCAGU- -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 28019 | 0.66 | 0.558982 |
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Target: 5'- ---cGGCCAGG-AguUGCCCUGGUaCAu -3' miRNA: 3'- gacaCCGGUCCaUguGUGGGACCA-GU- -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 6981 | 0.67 | 0.52686 |
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Target: 5'- ---cGGUagauGUACugGCCCUGGUCAu -3' miRNA: 3'- gacaCCGguc-CAUGugUGGGACCAGU- -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 10497 | 0.67 | 0.505831 |
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Target: 5'- -aGUGGCacgcaGGGUGCgACugUCUGGUg- -3' miRNA: 3'- gaCACCGg----UCCAUG-UGugGGACCAgu -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 9305 | 0.68 | 0.454965 |
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Target: 5'- ---gGGCCAGGUACGCAUcgaacagcgUCUGGcCGg -3' miRNA: 3'- gacaCCGGUCCAUGUGUG---------GGACCaGU- -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 32727 | 0.73 | 0.239477 |
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Target: 5'- -cGUcGGCCGGGUcCGCACCCUucacgaccacccacgGGUCGc -3' miRNA: 3'- gaCA-CCGGUCCAuGUGUGGGA---------------CCAGU- -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 50754 | 0.73 | 0.219063 |
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Target: 5'- -cGUcGGCCGGGggcgGCGCACCUUGGgcgCGu -3' miRNA: 3'- gaCA-CCGGUCCa---UGUGUGGGACCa--GU- -5' |
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| 1359 | 5' | -57 | NC_001335.1 | + | 18457 | 1.08 | 0.0007 |
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Target: 5'- uCUGUGGCCAGGUACACACCCUGGUCAc -3' miRNA: 3'- -GACACCGGUCCAUGUGUGGGACCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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