Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13607 | 3' | -56.6 | NC_003460.1 | + | 649 | 0.66 | 0.142729 |
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Target: 5'- gCUCgggUugGUCGGGUGcuuGGGGCGgccaaGCg -3' miRNA: 3'- -GGGa--AugCGGCCCGCu--UUCCGCag---CG- -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 4962 | 0.66 | 0.142729 |
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Target: 5'- aCUCUUG-GCgGGGCGAcgAAGGC--CGCu -3' miRNA: 3'- -GGGAAUgCGgCCCGCU--UUCCGcaGCG- -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 3826 | 0.66 | 0.125013 |
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Target: 5'- gCUCUUuCGgCGGGCGGgcagacugcGGGGCGgguguccUCGCu -3' miRNA: 3'- -GGGAAuGCgGCCCGCU---------UUCCGC-------AGCG- -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 434 | 0.69 | 0.079075 |
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Target: 5'- uCCCgu-CGUCGGGuCGgcGGGUGUUGa -3' miRNA: 3'- -GGGaauGCGGCCC-GCuuUCCGCAGCg -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 2274 | 0.7 | 0.066893 |
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Target: 5'- gCCCgugACGC--GGUGAAGGGUGcCGCg -3' miRNA: 3'- -GGGaa-UGCGgcCCGCUUUCCGCaGCG- -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 4740 | 0.7 | 0.066893 |
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Target: 5'- gUCCaUGCGCUgguccuccuGGGCGAGGGuGCGcgaggUCGCg -3' miRNA: 3'- -GGGaAUGCGG---------CCCGCUUUC-CGC-----AGCG- -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 5390 | 0.72 | 0.046138 |
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Target: 5'- aUCgacCGCCuGGCGAGAGGCGcuuagCGCa -3' miRNA: 3'- gGGaauGCGGcCCGCUUUCCGCa----GCG- -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 222 | 0.74 | 0.027655 |
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Target: 5'- gCCUUuCGCCcGGCGuAAGGGCGgCGCu -3' miRNA: 3'- gGGAAuGCGGcCCGC-UUUCCGCaGCG- -5' |
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| 13607 | 3' | -56.6 | NC_003460.1 | + | 248 | 1.14 | 1.2e-05 |
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Target: 5'- gCCCUUACGCCGGGCGAAAGGCGUCGCg -3' miRNA: 3'- -GGGAAUGCGGCCCGCUUUCCGCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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