Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13608 | 5' | -59.2 | NC_003460.1 | + | 592 | 1.1 | 0.000009 |
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Target: 5'- aCACCGCCACCAGCGGCGACAGCAAGGa -3' miRNA: 3'- -GUGGCGGUGGUCGCCGCUGUCGUUCC- -5' |
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| 13608 | 5' | -59.2 | NC_003460.1 | + | 887 | 0.7 | 0.033795 |
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Target: 5'- gCACCGgCAaCGGCGaCGAgGGCGAGGa -3' miRNA: 3'- -GUGGCgGUgGUCGCcGCUgUCGUUCC- -5' |
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| 13608 | 5' | -59.2 | NC_003460.1 | + | 4981 | 0.69 | 0.046505 |
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Target: 5'- uCGCC-CCGCCaagagucaauucaaaAGCGGCcuuagagaaguaGGCGGCGAGGc -3' miRNA: 3'- -GUGGcGGUGG---------------UCGCCG------------CUGUCGUUCC- -5' |
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| 13608 | 5' | -59.2 | NC_003460.1 | + | 3525 | 0.68 | 0.056183 |
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Target: 5'- -cCCGCaggggCACCcacAGCGGaugGGCGGCAAGGu -3' miRNA: 3'- guGGCG-----GUGG---UCGCCg--CUGUCGUUCC- -5' |
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| 13608 | 5' | -59.2 | NC_003460.1 | + | 1773 | 0.67 | 0.068716 |
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Target: 5'- uCGCCGCCcCCA-UGGCGAaauuGCAaAGGg -3' miRNA: 3'- -GUGGCGGuGGUcGCCGCUgu--CGU-UCC- -5' |
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| 13608 | 5' | -59.2 | NC_003460.1 | + | 2517 | 0.72 | 0.024016 |
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Target: 5'- gGCCaggGCgGCCagGGCGGCcagGGCGGCAGGGg -3' miRNA: 3'- gUGG---CGgUGG--UCGCCG---CUGUCGUUCC- -5' |
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| 13608 | 5' | -59.2 | NC_003460.1 | + | 291 | 0.7 | 0.034966 |
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Target: 5'- gACUGCUucGCgAGCGGUGGCGGCugcuuAAGGc -3' miRNA: 3'- gUGGCGG--UGgUCGCCGCUGUCG-----UUCC- -5' |
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| 13608 | 5' | -59.2 | NC_003460.1 | + | 2481 | 0.69 | 0.04588 |
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Target: 5'- aGCCGU----GGCGGCGAUgguGGCAAGGg -3' miRNA: 3'- gUGGCGguggUCGCCGCUG---UCGUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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