Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13609 | 3' | -52.9 | NC_003460.1 | + | 2135 | 0.66 | 0.230472 |
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Target: 5'- gGCGCCGG---UCGGUGGgacugaCUGUGc -3' miRNA: 3'- gUGCGGCUauuAGCCGCCaua---GACGC- -5' |
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| 13609 | 3' | -52.9 | NC_003460.1 | + | 1944 | 0.72 | 0.082214 |
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Target: 5'- cCugGCgGAUGGgacggCGGCGG-GUUUGCGg -3' miRNA: 3'- -GugCGgCUAUUa----GCCGCCaUAGACGC- -5' |
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| 13609 | 3' | -52.9 | NC_003460.1 | + | 3780 | 0.72 | 0.076727 |
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Target: 5'- gCAuCGCCuGAUcauGUCcagGGUGGUGUCUGCGg -3' miRNA: 3'- -GU-GCGG-CUAu--UAG---CCGCCAUAGACGC- -5' |
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| 13609 | 3' | -52.9 | NC_003460.1 | + | 4238 | 0.72 | 0.069148 |
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Target: 5'- aCACGCCGAUGggucgaaucgccGUCGGCGuGUccgaugGUCaGCGa -3' miRNA: 3'- -GUGCGGCUAU------------UAGCCGC-CA------UAGaCGC- -5' |
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| 13609 | 3' | -52.9 | NC_003460.1 | + | 1344 | 0.73 | 0.060157 |
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Target: 5'- uCACGCCGAUuAUCGGCGuGgcUAUCgGUGu -3' miRNA: 3'- -GUGCGGCUAuUAGCCGC-C--AUAGaCGC- -5' |
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| 13609 | 3' | -52.9 | NC_003460.1 | + | 1369 | 0.93 | 0.001315 |
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Target: 5'- cCACGCCGAUAAUCGGCGuG-AUCUGCGg -3' miRNA: 3'- -GUGCGGCUAUUAGCCGC-CaUAGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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