Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13612 | 5' | -55.6 | NC_003460.1 | + | 2488 | 0.67 | 0.131184 |
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Target: 5'- -gCGGCGAUgGUggcaagGGCGgCGACGGCg -3' miRNA: 3'- ggGCCGUUAgCGa-----CUGUgGCUGCUGa -5' |
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| 13612 | 5' | -55.6 | NC_003460.1 | + | 2882 | 0.67 | 0.115032 |
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Target: 5'- gCCCGGCGgcggugAUCGCUGAuguuCGCC--UGGCUg -3' miRNA: 3'- -GGGCCGU------UAGCGACU----GUGGcuGCUGA- -5' |
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| 13612 | 5' | -55.6 | NC_003460.1 | + | 473 | 0.67 | 0.115032 |
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Target: 5'- aCCaCGGCucaacaccCGCcGAC-CCGACGACg -3' miRNA: 3'- -GG-GCCGuua-----GCGaCUGuGGCUGCUGa -5' |
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| 13612 | 5' | -55.6 | NC_003460.1 | + | 229 | 0.68 | 0.097452 |
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Target: 5'- gCCCGGCGuaagggcggCGCUGggauucaauACACCGcgcuGCGGCg -3' miRNA: 3'- -GGGCCGUua-------GCGAC---------UGUGGC----UGCUGa -5' |
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| 13612 | 5' | -55.6 | NC_003460.1 | + | 1070 | 0.69 | 0.094254 |
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Target: 5'- cCCCGGUccAUCGUcacCACUGACGACa -3' miRNA: 3'- -GGGCCGu-UAGCGacuGUGGCUGCUGa -5' |
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| 13612 | 5' | -55.6 | NC_003460.1 | + | 4310 | 0.7 | 0.074509 |
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Target: 5'- aUCCGGUccacAAggugcgccUCGCUGACcaucggacacGCCGACGGCg -3' miRNA: 3'- -GGGCCG----UU--------AGCGACUG----------UGGCUGCUGa -5' |
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| 13612 | 5' | -55.6 | NC_003460.1 | + | 3274 | 0.71 | 0.058765 |
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Target: 5'- uCCUGGCAAUCGaagcgugauCUG-CGCCGGCGGu- -3' miRNA: 3'- -GGGCCGUUAGC---------GACuGUGGCUGCUga -5' |
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| 13612 | 5' | -55.6 | NC_003460.1 | + | 2012 | 1.1 | 3.2e-05 |
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Target: 5'- uCCCGGCAAUCGCUGACACCGACGACUg -3' miRNA: 3'- -GGGCCGUUAGCGACUGUGGCUGCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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