Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13625 | 5' | -56.1 | NC_003460.1 | + | 2513 | 0.74 | 0.02292 |
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Target: 5'- cGgcGGCCAGGgcGGCCaggGCgGCCAGGGCg -3' miRNA: 3'- aCuuCCGGUUC--UCGG---CGgUGGUUCUG- -5' |
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| 13625 | 5' | -56.1 | NC_003460.1 | + | 5670 | 0.71 | 0.040366 |
|
Target: 5'- aGggGuGCgCGGGAGgcCCGCCGCCA-GGCg -3' miRNA: 3'- aCuuC-CG-GUUCUC--GGCGGUGGUuCUG- -5' |
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| 13625 | 5' | -56.1 | NC_003460.1 | + | 2931 | 0.67 | 0.092992 |
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Target: 5'- cGAAGGCCuggacGGGGCUGUUGgCGAGGg -3' miRNA: 3'- aCUUCCGGu----UCUCGGCGGUgGUUCUg -5' |
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| 13625 | 5' | -56.1 | NC_003460.1 | + | 693 | 0.66 | 0.11796 |
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Target: 5'- cGcuGGCUcuGGGUCGUCGCCucGACg -3' miRNA: 3'- aCuuCCGGuuCUCGGCGGUGGuuCUG- -5' |
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| 13625 | 5' | -56.1 | NC_003460.1 | + | 5379 | 1.09 | 0.000022 |
|
Target: 5'- uUGAAGGCCAAGAGCCGCCACCAAGACc -3' miRNA: 3'- -ACUUCCGGUUCUCGGCGGUGGUUCUG- -5' |
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| 13625 | 5' | -56.1 | NC_003460.1 | + | 607 | 0.68 | 0.075659 |
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Target: 5'- aUGAuGGCgGGGGugacaCCGCCACCAGcGGCg -3' miRNA: 3'- -ACUuCCGgUUCUc----GGCGGUGGUU-CUG- -5' |
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| 13625 | 5' | -56.1 | NC_003460.1 | + | 3021 | 0.68 | 0.081062 |
|
Target: 5'- -cGAGGCCAccgaucccgcuAGAGaCGCCAUCGGGgACa -3' miRNA: 3'- acUUCCGGU-----------UCUCgGCGGUGGUUC-UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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