Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
13627 | 5' | -61.7 | NC_003460.1 | + | 1314 | 0.66 | 0.05243 |
Target: 5'- cUGCUGCGaucacggcuCUcGGGCCgcagaucACGCCgauuaucGGCGUGGc -3' miRNA: 3'- -ACGACGC---------GA-CCUGG-------UGCGG-------CCGCACC- -5' |
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13627 | 5' | -61.7 | NC_003460.1 | + | 2115 | 0.71 | 0.01806 |
Target: 5'- gGuCUGCcCUGGACUcccucgGCGCCGGUcgGUGGg -3' miRNA: 3'- aC-GACGcGACCUGG------UGCGGCCG--CACC- -5' |
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13627 | 5' | -61.7 | NC_003460.1 | + | 241 | 0.74 | 0.009494 |
Target: 5'- gGCgGCGCUGGgauucaauacACCGCGCugCGGCGUGu -3' miRNA: 3'- aCGaCGCGACC----------UGGUGCG--GCCGCACc -5' |
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13627 | 5' | -61.7 | NC_003460.1 | + | 3728 | 0.76 | 0.007336 |
Target: 5'- cGCuUGCGUcgucucgcggaUGaucaggucagcacgaGACCACGCCGGCGUGGu -3' miRNA: 3'- aCG-ACGCG-----------AC---------------CUGGUGCGGCCGCACC- -5' |
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13627 | 5' | -61.7 | NC_003460.1 | + | 3658 | 1.09 | 6e-06 |
Target: 5'- gUGCUGCGCUGGACCACGCCGGCGUGGu -3' miRNA: 3'- -ACGACGCGACCUGGUGCGGCCGCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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