Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1365 | 5' | -51.9 | NC_001335.1 | + | 48100 | 0.66 | 0.881908 |
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Target: 5'- gGACGCCGGa----AUCGGaGAGGUa- -3' miRNA: 3'- gCUGCGGUCguuagUAGCC-CUCUAac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 38880 | 0.66 | 0.865855 |
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Target: 5'- uGAaccCGCCGGCcguccaAGUCAUCGGG-GAg-- -3' miRNA: 3'- gCU---GCGGUCG------UUAGUAGCCCuCUaac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 44379 | 0.67 | 0.839867 |
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Target: 5'- uCGugGCCAGaCGA-CAUCGaGGAGc--- -3' miRNA: 3'- -GCugCGGUC-GUUaGUAGC-CCUCuaac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 5856 | 0.67 | 0.821374 |
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Target: 5'- gCGugGCCAGCGGgccgagcUGGGGGAg-- -3' miRNA: 3'- -GCugCGGUCGUUagua---GCCCUCUaac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 45733 | 0.69 | 0.718147 |
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Target: 5'- -uGCGCCAGUucAUCGUUGGcGAGAUc- -3' miRNA: 3'- gcUGCGGUCGu-UAGUAGCC-CUCUAac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 29170 | 0.7 | 0.662382 |
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Target: 5'- -uGCGUCAGCGGUCAUCugguGGAGGUc- -3' miRNA: 3'- gcUGCGGUCGUUAGUAGc---CCUCUAac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 26142 | 0.72 | 0.561001 |
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Target: 5'- uCGACGCCAGUAGcCAgagCGGaGGGAUc- -3' miRNA: 3'- -GCUGCGGUCGUUaGUa--GCC-CUCUAac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 21985 | 0.76 | 0.350479 |
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Target: 5'- aCGAUGCCAGCGGUCA-CGauGGAGAa-- -3' miRNA: 3'- -GCUGCGGUCGUUAGUaGC--CCUCUaac -5' |
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| 1365 | 5' | -51.9 | NC_001335.1 | + | 21270 | 1.08 | 0.002309 |
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Target: 5'- cCGACGCCAGCAAUCAUCGGGAGAUUGc -3' miRNA: 3'- -GCUGCGGUCGUUAGUAGCCCUCUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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