Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1368 | 5' | -50.9 | NC_001335.1 | + | 11854 | 0.66 | 0.909698 |
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Target: 5'- uUGGUgGUCUgcgcuaggaaGGUCGGgAACGGGcUGu -3' miRNA: 3'- -ACUAgCAGAa---------CCAGCUgUUGCCCuAC- -5' |
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| 1368 | 5' | -50.9 | NC_001335.1 | + | 37530 | 0.67 | 0.856077 |
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Target: 5'- gGAUCGgCUugugGGUgucagugaCGACGACGGGAa- -3' miRNA: 3'- aCUAGCaGAa---CCA--------GCUGUUGCCCUac -5' |
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| 1368 | 5' | -50.9 | NC_001335.1 | + | 7730 | 0.7 | 0.748843 |
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Target: 5'- gUGAUCGgguUCUUGGUCGACAGaauCGacuuGAUGa -3' miRNA: 3'- -ACUAGC---AGAACCAGCUGUU---GCc---CUAC- -5' |
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| 1368 | 5' | -50.9 | NC_001335.1 | + | 29927 | 0.7 | 0.705243 |
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Target: 5'- uUGGUCGUCUUGuGUCGGCGGCu----- -3' miRNA: 3'- -ACUAGCAGAAC-CAGCUGUUGcccuac -5' |
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| 1368 | 5' | -50.9 | NC_001335.1 | + | 31886 | 0.77 | 0.348802 |
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Target: 5'- aGGUCGUCUcGGUCGu---CGGGGUGg -3' miRNA: 3'- aCUAGCAGAaCCAGCuguuGCCCUAC- -5' |
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| 1368 | 5' | -50.9 | NC_001335.1 | + | 23892 | 1.09 | 0.003016 |
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Target: 5'- gUGAUCGUCUUGGUCGACAACGGGAUGu -3' miRNA: 3'- -ACUAGCAGAACCAGCUGUUGCCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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