Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1369 | 5' | -57.9 | NC_001335.1 | + | 11724 | 0.66 | 0.583033 |
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Target: 5'- -gGACGGuUUCCGCCgaggccacgaagauCGUCGCGa- -3' miRNA: 3'- caCUGCC-AAGGCGGagu-----------GCAGCGCcu -5' |
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| 1369 | 5' | -57.9 | NC_001335.1 | + | 31769 | 0.66 | 0.581965 |
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Target: 5'- cGUGGCGGUacgucaucaaggcCCGCUggCACGaggcCGCGGAu -3' miRNA: 3'- -CACUGCCAa------------GGCGGa-GUGCa---GCGCCU- -5' |
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| 1369 | 5' | -57.9 | NC_001335.1 | + | 4636 | 0.67 | 0.54384 |
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Target: 5'- -cGACGGUcUCC-CCUaCGCGUacgGCGGGg -3' miRNA: 3'- caCUGCCA-AGGcGGA-GUGCAg--CGCCU- -5' |
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| 1369 | 5' | -57.9 | NC_001335.1 | + | 46773 | 0.73 | 0.233247 |
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Target: 5'- -aGACGGUguacgCCaGCCUCACGggcggucaGCGGAu -3' miRNA: 3'- caCUGCCAa----GG-CGGAGUGCag------CGCCU- -5' |
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| 1369 | 5' | -57.9 | NC_001335.1 | + | 24641 | 1.08 | 0.000656 |
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Target: 5'- uGUGACGGUUCCGCCUCACGUCGCGGAg -3' miRNA: 3'- -CACUGCCAAGGCGGAGUGCAGCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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