Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1370 | 3' | -56 | NC_001335.1 | + | 17020 | 0.67 | 0.594133 |
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Target: 5'- cGAGcGUGcuCGCCAGCgUCUGGaaggccgGCGUGa -3' miRNA: 3'- -CUC-UACu-GCGGUCG-AGACCa------CGCACg -5' |
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| 1370 | 3' | -56 | NC_001335.1 | + | 8600 | 0.67 | 0.583119 |
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Target: 5'- -cGGUGACGCCgauGGcCUCUGGccgGCGcucaGCg -3' miRNA: 3'- cuCUACUGCGG---UC-GAGACCa--CGCa---CG- -5' |
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| 1370 | 3' | -56 | NC_001335.1 | + | 43256 | 0.69 | 0.436576 |
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Target: 5'- cGGGUGACGacuuCCAGCUCg---GCGUGUa -3' miRNA: 3'- cUCUACUGC----GGUCGAGaccaCGCACG- -5' |
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| 1370 | 3' | -56 | NC_001335.1 | + | 17322 | 0.7 | 0.417342 |
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Target: 5'- ---cUGGCGCaGGCUCUGGUcaGCGUuGCu -3' miRNA: 3'- cucuACUGCGgUCGAGACCA--CGCA-CG- -5' |
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| 1370 | 3' | -56 | NC_001335.1 | + | 17633 | 0.73 | 0.255905 |
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Target: 5'- gGAGcUGGCGuCCAGCguggCUGGUGCaGUGa -3' miRNA: 3'- -CUCuACUGC-GGUCGa---GACCACG-CACg -5' |
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| 1370 | 3' | -56 | NC_001335.1 | + | 42701 | 0.74 | 0.24229 |
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Target: 5'- cGGGAUGACGCaCGGcCUCcgcuUGGgcaaaccUGCGUGCa -3' miRNA: 3'- -CUCUACUGCG-GUC-GAG----ACC-------ACGCACG- -5' |
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| 1370 | 3' | -56 | NC_001335.1 | + | 25055 | 1.12 | 0.000414 |
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Target: 5'- aGAGAUGACGCCAGCUCUGGUGCGUGCg -3' miRNA: 3'- -CUCUACUGCGGUCGAGACCACGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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