Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1372 | 5' | -54.5 | NC_001335.1 | + | 14302 | 0.66 | 0.731011 |
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Target: 5'- gGCUacGugUACgUCGGUGcugcaGGCACCGc -3' miRNA: 3'- -CGA--CugAUGgAGCCACag---UCGUGGUu -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 36922 | 0.66 | 0.720151 |
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Target: 5'- --gGACUACCcgagcccgucUCGGUGgaccaagGGCACCAAg -3' miRNA: 3'- cgaCUGAUGG----------AGCCACag-----UCGUGGUU- -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 4205 | 0.66 | 0.685962 |
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Target: 5'- uGCgGugUuACCUCGGcUGUCGGUaaacgcgGCCGAg -3' miRNA: 3'- -CGaCugA-UGGAGCC-ACAGUCG-------UGGUU- -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 1699 | 0.67 | 0.61974 |
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Target: 5'- cGCUccGACagccgGCCUCGGcaUGUacggCGGCACCAAg -3' miRNA: 3'- -CGA--CUGa----UGGAGCC--ACA----GUCGUGGUU- -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 5289 | 0.68 | 0.597272 |
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Target: 5'- cGCaGGCgAUCUCGGUGagCuGCACCGAa -3' miRNA: 3'- -CGaCUGaUGGAGCCACa-GuCGUGGUU- -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 15790 | 0.68 | 0.587197 |
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Target: 5'- uGCUGGCguagaagucgcccggAUCUCGGUcaagGUCAGC-CCGAa -3' miRNA: 3'- -CGACUGa--------------UGGAGCCA----CAGUCGuGGUU- -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 28695 | 0.69 | 0.498688 |
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Target: 5'- cGCUuuCcgACCUCGGgGUCGGUGCCAAc -3' miRNA: 3'- -CGAcuGa-UGGAGCCaCAGUCGUGGUU- -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 4154 | 0.7 | 0.477708 |
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Target: 5'- cGCcGGCUGCCUCGccgGUCGGCaugugaACCAAc -3' miRNA: 3'- -CGaCUGAUGGAGCca-CAGUCG------UGGUU- -5' |
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| 1372 | 5' | -54.5 | NC_001335.1 | + | 26723 | 1.09 | 0.000988 |
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Target: 5'- cGCUGACUACCUCGGUGUCAGCACCAAg -3' miRNA: 3'- -CGACUGAUGGAGCCACAGUCGUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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