Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1378 | 3' | -58.2 | NC_001335.1 | + | 32967 | 0.66 | 0.516302 |
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Target: 5'- aUGgGGcaugCGCCGUUGGCGCaCUCu- -3' miRNA: 3'- cACgCCua--GCGGUAGCCGCGaGAGuu -5' |
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| 1378 | 3' | -58.2 | NC_001335.1 | + | 52130 | 0.68 | 0.379774 |
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Target: 5'- -aGCcGcgUGCCG-CGGCGCUCUCGc -3' miRNA: 3'- caCGcCuaGCGGUaGCCGCGAGAGUu -5' |
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| 1378 | 3' | -58.2 | NC_001335.1 | + | 9787 | 0.69 | 0.32911 |
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Target: 5'- uUGCGGccucuuccucGUC-CCAUCGGCGCaUCUCu- -3' miRNA: 3'- cACGCC----------UAGcGGUAGCCGCG-AGAGuu -5' |
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| 1378 | 3' | -58.2 | NC_001335.1 | + | 17379 | 0.7 | 0.269595 |
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Target: 5'- -gGCGGcgCGCuCAUCGGCGCgcuggcgaacCUCAc -3' miRNA: 3'- caCGCCuaGCG-GUAGCCGCGa---------GAGUu -5' |
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| 1378 | 3' | -58.2 | NC_001335.1 | + | 19498 | 0.73 | 0.196969 |
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Target: 5'- -gGCGGAagUCGCCAUCGcGCaGCUCUa-- -3' miRNA: 3'- caCGCCU--AGCGGUAGC-CG-CGAGAguu -5' |
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| 1378 | 3' | -58.2 | NC_001335.1 | + | 30256 | 1.06 | 0.00064 |
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Target: 5'- cGUGCGGAUCGCCAUCGGCGCUCUCAAg -3' miRNA: 3'- -CACGCCUAGCGGUAGCCGCGAGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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