Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 138 | 3' | -63.4 | AC_000006.1 | + | 16031 | 0.71 | 0.100272 |
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Target: 5'- cCGCuCUGCCGCCuGGUCCAGGAUg--- -3' miRNA: 3'- -GCG-GGCGGCGGuCCGGGUCUUGaccg -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 10006 | 0.71 | 0.094833 |
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Target: 5'- gCGCCCGggGCCAGGUCCucGAGCaugaGGCg -3' miRNA: 3'- -GCGGGCggCGGUCCGGGu-CUUGa---CCG- -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 9637 | 0.71 | 0.089675 |
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Target: 5'- aGCCgGUgucaggGCCAGGCCUGGGGCaUGGCc -3' miRNA: 3'- gCGGgCGg-----CGGUCCGGGUCUUG-ACCG- -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 10052 | 0.71 | 0.089675 |
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Target: 5'- gGCCCcggGCgCGCCGGccaCCCAGcGCUGGCc -3' miRNA: 3'- gCGGG---CG-GCGGUCc--GGGUCuUGACCG- -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 16854 | 0.72 | 0.087196 |
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Target: 5'- gGCCCGCCGCCGgcggcGGUUCAGGccGCUGccuGCc -3' miRNA: 3'- gCGGGCGGCGGU-----CCGGGUCU--UGAC---CG- -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 5313 | 0.73 | 0.073644 |
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Target: 5'- gGCCaCGCCGCCgAGGCCCucaacuacGAGCUcauGGUc -3' miRNA: 3'- gCGG-GCGGCGG-UCCGGGu-------CUUGA---CCG- -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 8044 | 0.8 | 0.019622 |
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Target: 5'- aGCCCGCgcgggaGCCAGGUCCAGAuCUcGGCg -3' miRNA: 3'- gCGGGCGg-----CGGUCCGGGUCUuGA-CCG- -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 25548 | 0.83 | 0.010333 |
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Target: 5'- gCGCCCGCgGCCAGGCCCAu--CUGGg -3' miRNA: 3'- -GCGGGCGgCGGUCCGGGUcuuGACCg -5' |
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| 138 | 3' | -63.4 | AC_000006.1 | + | 17605 | 1.11 | 0.000059 |
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Target: 5'- cCGCCCGCCGCCAGGCCCAGAACUGGCa -3' miRNA: 3'- -GCGGGCGGCGGUCCGGGUCUUGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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