Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1381 | 3' | -51.7 | NC_001335.1 | + | 25367 | 0.66 | 0.840906 |
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Target: 5'- cAAGG-CUACCGGGUUGC-AACUccugUGCa -3' miRNA: 3'- -UUCCuGAUGGCCUAGUGcUUGA----ACGa -5' |
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| 1381 | 3' | -51.7 | NC_001335.1 | + | 36158 | 0.67 | 0.822213 |
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Target: 5'- -cGGACUucGCUGGAggugaucuggCGCGAACgUGCg -3' miRNA: 3'- uuCCUGA--UGGCCUa---------GUGCUUGaACGa -5' |
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| 1381 | 3' | -51.7 | NC_001335.1 | + | 1364 | 0.67 | 0.812535 |
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Target: 5'- gGGGGGC-ACCGGGgugUugGGGCU-GCUg -3' miRNA: 3'- -UUCCUGaUGGCCUa--GugCUUGAaCGA- -5' |
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| 1381 | 3' | -51.7 | NC_001335.1 | + | 24830 | 0.67 | 0.792575 |
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Target: 5'- cGAGcACUACCGGAUCGCGGcauACauccugGCg -3' miRNA: 3'- -UUCcUGAUGGCCUAGUGCU---UGaa----CGa -5' |
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| 1381 | 3' | -51.7 | NC_001335.1 | + | 9505 | 0.69 | 0.695237 |
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Target: 5'- -cGGAucgcCUACCGGAUCAUGAAgg-GCg -3' miRNA: 3'- uuCCU----GAUGGCCUAGUGCUUgaaCGa -5' |
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| 1381 | 3' | -51.7 | NC_001335.1 | + | 31312 | 1.05 | 0.00349 |
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Target: 5'- cAAGGACUACCGGAUCACGAACUUGCUc -3' miRNA: 3'- -UUCCUGAUGGCCUAGUGCUUGAACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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