Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1386 | 3' | -52.9 | NC_001335.1 | + | 24354 | 0.66 | 0.781954 |
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Target: 5'- aUACCAGAUGgcguCUGaGGCccGCGACGa -3' miRNA: 3'- gAUGGUCUACac--GAC-CCGacUGUUGC- -5' |
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| 1386 | 3' | -52.9 | NC_001335.1 | + | 6027 | 0.67 | 0.771659 |
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Target: 5'- gCUGCCGGGUaGuUGCcgaUGGGCUGcCAcCGg -3' miRNA: 3'- -GAUGGUCUA-C-ACG---ACCCGACuGUuGC- -5' |
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| 1386 | 3' | -52.9 | NC_001335.1 | + | 9512 | 0.67 | 0.761211 |
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Target: 5'- cCUACCGGAUcaUGaaGGGC-GGCGACGu -3' miRNA: 3'- -GAUGGUCUAc-ACgaCCCGaCUGUUGC- -5' |
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| 1386 | 3' | -52.9 | NC_001335.1 | + | 38073 | 0.67 | 0.739909 |
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Target: 5'- -cGCCgAGcUGUG-UGGGCUGcCGACGg -3' miRNA: 3'- gaUGG-UCuACACgACCCGACuGUUGC- -5' |
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| 1386 | 3' | -52.9 | NC_001335.1 | + | 6512 | 0.68 | 0.696025 |
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Target: 5'- -aGCUcGAUGaGCUGGGC-GAUGACGg -3' miRNA: 3'- gaUGGuCUACaCGACCCGaCUGUUGC- -5' |
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| 1386 | 3' | -52.9 | NC_001335.1 | + | 5852 | 0.7 | 0.594585 |
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Target: 5'- -gGCCAGcgGgccgaGCUGGgggagagaaccGCUGACGGCGg -3' miRNA: 3'- gaUGGUCuaCa----CGACC-----------CGACUGUUGC- -5' |
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| 1386 | 3' | -52.9 | NC_001335.1 | + | 40254 | 0.71 | 0.495666 |
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Target: 5'- -cGCCAGcagGUGCUGGGCgcucUGAUcaaGACGa -3' miRNA: 3'- gaUGGUCua-CACGACCCG----ACUG---UUGC- -5' |
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| 1386 | 3' | -52.9 | NC_001335.1 | + | 33271 | 1.08 | 0.001698 |
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Target: 5'- uCUACCAGAUGUGCUGGGCUGACAACGc -3' miRNA: 3'- -GAUGGUCUACACGACCCGACUGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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