Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1387 | 3' | -53.7 | NC_001335.1 | + | 30048 | 0.66 | 0.807531 |
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Target: 5'- gGGCCGGG-GGUccaccagcuUCGCCag-GAUCUGc -3' miRNA: 3'- -CCGGCUUgCCA---------AGCGGguaCUAGACu -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 8984 | 0.66 | 0.797879 |
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Target: 5'- gGGCUGAGUGGUUCaacguCCCGcacGGUCUGGg -3' miRNA: 3'- -CCGGCUUGCCAAGc----GGGUa--CUAGACU- -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 17023 | 0.66 | 0.778059 |
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Target: 5'- uGCCGAGCGuGcUCGCCagc-GUCUGGa -3' miRNA: 3'- cCGGCUUGC-CaAGCGGguacUAGACU- -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 33855 | 0.68 | 0.697938 |
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Target: 5'- uGGCCGAgguugcacggcucccACGGUUCGagaGUGAUCUc- -3' miRNA: 3'- -CCGGCU---------------UGCCAAGCgggUACUAGAcu -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 9497 | 0.69 | 0.605181 |
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Target: 5'- aGGCCauGCGGaUCGCCUAccgGAUCaUGAa -3' miRNA: 3'- -CCGGcuUGCCaAGCGGGUa--CUAG-ACU- -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 16672 | 0.7 | 0.572145 |
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Target: 5'- uGCCGAACGaGUUCGCgCcgGcgUUGGa -3' miRNA: 3'- cCGGCUUGC-CAAGCGgGuaCuaGACU- -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 10320 | 0.7 | 0.550357 |
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Target: 5'- gGGCCGGAUcGUUcuugacagCGCCgAUGAUCUGu -3' miRNA: 3'- -CCGGCUUGcCAA--------GCGGgUACUAGACu -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 21669 | 0.72 | 0.45631 |
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Target: 5'- cGCCGAGCaGUgucacguagUCGCCUAUGGagUCUGGg -3' miRNA: 3'- cCGGCUUGcCA---------AGCGGGUACU--AGACU- -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 38345 | 0.72 | 0.426895 |
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Target: 5'- cGGCCGAGCGGaacuaaGCCguUcGGUCUGGg -3' miRNA: 3'- -CCGGCUUGCCaag---CGGguA-CUAGACU- -5' |
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| 1387 | 3' | -53.7 | NC_001335.1 | + | 33627 | 1.12 | 0.000962 |
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Target: 5'- aGGCCGAACGGUUCGCCCAUGAUCUGAg -3' miRNA: 3'- -CCGGCUUGCCAAGCGGGUACUAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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