Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1389 | 5' | -60 | NC_001335.1 | + | 48367 | 0.7 | 0.289429 |
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Target: 5'- gGUGugGuUCGGCCGGCcGGcCgCGACa -3' miRNA: 3'- gCACugC-GGCCGGCCGuUCuG-GCUGa -5' |
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| 1389 | 5' | -60 | NC_001335.1 | + | 50921 | 0.7 | 0.282473 |
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Target: 5'- gGUGACGcCCGGCCugucGGCcuGGCUGAa- -3' miRNA: 3'- gCACUGC-GGCCGG----CCGuuCUGGCUga -5' |
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| 1389 | 5' | -60 | NC_001335.1 | + | 34270 | 0.71 | 0.249673 |
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Target: 5'- gGUGuGCGCCGGUCGGCGcGGAugUCGAUg -3' miRNA: 3'- gCAC-UGCGGCCGGCCGU-UCU--GGCUGa -5' |
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| 1389 | 5' | -60 | NC_001335.1 | + | 22588 | 0.71 | 0.249673 |
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Target: 5'- aCGUGGUGCCGGCCaGCuc-GCCGAUa -3' miRNA: 3'- -GCACUGCGGCCGGcCGuucUGGCUGa -5' |
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| 1389 | 5' | -60 | NC_001335.1 | + | 5601 | 0.73 | 0.183726 |
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Target: 5'- gGUG-CGCCGGCCguagcGGCAGGcACCGuACg -3' miRNA: 3'- gCACuGCGGCCGG-----CCGUUC-UGGC-UGa -5' |
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| 1389 | 5' | -60 | NC_001335.1 | + | 2329 | 0.74 | 0.144848 |
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Target: 5'- aGUGA-GCUgGGCCGGCucAGGCCGGCg -3' miRNA: 3'- gCACUgCGG-CCGGCCGu-UCUGGCUGa -5' |
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| 1389 | 5' | -60 | NC_001335.1 | + | 33888 | 1.07 | 0.000467 |
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Target: 5'- uCGUGACGCCGGCCGGCAAGACCGACUa -3' miRNA: 3'- -GCACUGCGGCCGGCCGUUCUGGCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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