Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1390 | 3' | -57.1 | NC_001335.1 | + | 23811 | 0.71 | 0.317971 |
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Target: 5'- cCCcAGCCgucaGCCAGGGCCAuGCCUu -3' miRNA: 3'- uGGcUUGGaug-CGGUCCUGGUuCGGA- -5' |
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| 1390 | 3' | -57.1 | NC_001335.1 | + | 14271 | 0.72 | 0.283629 |
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Target: 5'- cGCCGAGCCUgaucagacccuggauGCGCCAGGAguCCugguagaggGAGCUg -3' miRNA: 3'- -UGGCUUGGA---------------UGCGGUCCU--GG---------UUCGGa -5' |
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| 1390 | 3' | -57.1 | NC_001335.1 | + | 48303 | 0.72 | 0.273777 |
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Target: 5'- gGCCGAACCacacccUugGCguGGGCCAgcaggacguGGCCg -3' miRNA: 3'- -UGGCUUGG------AugCGguCCUGGU---------UCGGa -5' |
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| 1390 | 3' | -57.1 | NC_001335.1 | + | 9345 | 0.72 | 0.269639 |
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Target: 5'- uGCUGGAucuugcccucagcguCCUcgaACGCCAGGAuCCGGGCCa -3' miRNA: 3'- -UGGCUU---------------GGA---UGCGGUCCU-GGUUCGGa -5' |
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| 1390 | 3' | -57.1 | NC_001335.1 | + | 26151 | 0.74 | 0.211246 |
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Target: 5'- gGCCaGAACCcGCGCCucGGGGCCugGAGCCc -3' miRNA: 3'- -UGG-CUUGGaUGCGG--UCCUGG--UUCGGa -5' |
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| 1390 | 3' | -57.1 | NC_001335.1 | + | 19533 | 0.74 | 0.211246 |
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Target: 5'- cUCGAGCacccgcuCGCCAGcGACCAGGCCg -3' miRNA: 3'- uGGCUUGgau----GCGGUC-CUGGUUCGGa -5' |
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| 1390 | 3' | -57.1 | NC_001335.1 | + | 17390 | 0.78 | 0.097717 |
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Target: 5'- gACCaGAGCCUGCGCCAGGGgCAAaCCa -3' miRNA: 3'- -UGG-CUUGGAUGCGGUCCUgGUUcGGa -5' |
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| 1390 | 3' | -57.1 | NC_001335.1 | + | 35013 | 1.08 | 0.000722 |
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Target: 5'- aACCGAACCUACGCCAGGACCAAGCCUg -3' miRNA: 3'- -UGGCUUGGAUGCGGUCCUGGUUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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