Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1393 | 5' | -55.5 | NC_001335.1 | + | 28578 | 0.66 | 0.699387 |
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Target: 5'- cGUCgccUGCGGgaAGGuGGcgCACUCGGCGc -3' miRNA: 3'- -CAGaa-GCGCC--UCCuCCa-GUGAGCUGU- -5' |
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| 1393 | 5' | -55.5 | NC_001335.1 | + | 50413 | 0.66 | 0.692882 |
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Target: 5'- ---gUCGCGGGGccaccgugccuagucGAuagaGGUCACUCGACu -3' miRNA: 3'- cagaAGCGCCUC---------------CU----CCAGUGAGCUGu -5' |
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| 1393 | 5' | -55.5 | NC_001335.1 | + | 3649 | 0.67 | 0.611644 |
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Target: 5'- gGUCgaCGUGGAGauGAcGGUCAC-CGACAc -3' miRNA: 3'- -CAGaaGCGCCUC--CU-CCAGUGaGCUGU- -5' |
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| 1393 | 5' | -55.5 | NC_001335.1 | + | 6797 | 0.68 | 0.589688 |
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Target: 5'- cGUCUggcgacguugaUCGCGcucuccGAGGcAGGUCuuCUCGACAa -3' miRNA: 3'- -CAGA-----------AGCGC------CUCC-UCCAGu-GAGCUGU- -5' |
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| 1393 | 5' | -55.5 | NC_001335.1 | + | 29705 | 0.68 | 0.557043 |
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Target: 5'- -aCggCGUGGAGaAGGUCaACUCGACc -3' miRNA: 3'- caGaaGCGCCUCcUCCAG-UGAGCUGu -5' |
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| 1393 | 5' | -55.5 | NC_001335.1 | + | 36206 | 1.08 | 0.001119 |
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Target: 5'- gGUCUUCGCGGAGGAGGUCACUCGACAc -3' miRNA: 3'- -CAGAAGCGCCUCCUCCAGUGAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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