Results 1 - 20 of 61 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13944 | 3' | -57.1 | NC_003521.1 | + | 78736 | 0.66 | 0.94307 |
Target: 5'- aGCGgcCAGGaGUUGUCGuCGGGCAcggccacgaGGUCc -3' miRNA: 3'- -CGCa-GUCCaCAACGGC-GCUCGU---------CCAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 128372 | 0.66 | 0.94307 |
Target: 5'- -gGUCAGGUccacGUUG-CGCuccAGCGGGUCc -3' miRNA: 3'- cgCAGUCCA----CAACgGCGc--UCGUCCAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 207299 | 0.66 | 0.94263 |
Target: 5'- aCG-CAGGU-UUGCCGCcGAugaaacagggauaGCAGGUCu -3' miRNA: 3'- cGCaGUCCAcAACGGCG-CU-------------CGUCCAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 119610 | 0.66 | 0.941744 |
Target: 5'- aGCGUCucgugcugcAGGUGgacguuuacccacugGCCGCGGGCGuccGUCu -3' miRNA: 3'- -CGCAG---------UCCACaa-------------CGGCGCUCGUc--CAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 55370 | 0.66 | 0.938574 |
Target: 5'- cGCGUCucgccGGGUGcggaggUGaUCGcCGAGCGGGUg -3' miRNA: 3'- -CGCAG-----UCCACa-----AC-GGC-GCUCGUCCAg -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 57139 | 0.66 | 0.938574 |
Target: 5'- aGCGaUAGGUcucGUcgGCCacGCGcGGCAGGUCg -3' miRNA: 3'- -CGCaGUCCA---CAa-CGG--CGC-UCGUCCAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 123474 | 0.66 | 0.938574 |
Target: 5'- gGCGUCAGcuccGUGUccGUCGCcGGCGGGg- -3' miRNA: 3'- -CGCAGUC----CACAa-CGGCGcUCGUCCag -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 32253 | 0.66 | 0.938113 |
Target: 5'- aGCGUCAGGccccggaUGUcauacuUGCCGUGAGCc-GUg -3' miRNA: 3'- -CGCAGUCC-------ACA------ACGGCGCUCGucCAg -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 128320 | 0.66 | 0.938113 |
Target: 5'- cCGU--GGUGUaGCCGCGguccucgGGCAGGUa -3' miRNA: 3'- cGCAguCCACAaCGGCGC-------UCGUCCAg -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 141976 | 0.66 | 0.933858 |
Target: 5'- aGCGUCAGGa---GCUGC-AGCGGGa- -3' miRNA: 3'- -CGCAGUCCacaaCGGCGcUCGUCCag -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 61621 | 0.66 | 0.928919 |
Target: 5'- uUG-CAGGaagcggGCCGUGGGCAGGUg -3' miRNA: 3'- cGCaGUCCacaa--CGGCGCUCGUCCAg -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 18543 | 0.66 | 0.928919 |
Target: 5'- uGCGgugCGGcccucccGUCGCGGGCAGGUCc -3' miRNA: 3'- -CGCa--GUCcacaa--CGGCGCUCGUCCAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 60869 | 0.66 | 0.928919 |
Target: 5'- gGCGUU-GGUGgUGCCGCGcguGUGGGcCu -3' miRNA: 3'- -CGCAGuCCACaACGGCGCu--CGUCCaG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 67565 | 0.66 | 0.923756 |
Target: 5'- uGUGUCAGGcGgcggGCCccGCGGGcCAGaGUCg -3' miRNA: 3'- -CGCAGUCCaCaa--CGG--CGCUC-GUC-CAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 130579 | 0.66 | 0.918371 |
Target: 5'- aGCGcCAGGUGcUGUacuucaaGCu-GCAGGUCa -3' miRNA: 3'- -CGCaGUCCACaACGg------CGcuCGUCCAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 123291 | 0.66 | 0.918371 |
Target: 5'- aGCGUCAGGU----CCGCGGGCgacucGGcGUCc -3' miRNA: 3'- -CGCAGUCCAcaacGGCGCUCG-----UC-CAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 103048 | 0.66 | 0.918371 |
Target: 5'- uGCG-CAGGUGcUGCaGacaGGGCGGGUg -3' miRNA: 3'- -CGCaGUCCACaACGgCg--CUCGUCCAg -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 121293 | 0.66 | 0.918371 |
Target: 5'- gGCGaCgAGGUcGgcGCCGcCGAGgAGGUCu -3' miRNA: 3'- -CGCaG-UCCA-CaaCGGC-GCUCgUCCAG- -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 213611 | 0.67 | 0.912764 |
Target: 5'- gGCuGUUGGGguggcgGUcGCCGCGcAGCAGGa- -3' miRNA: 3'- -CG-CAGUCCa-----CAaCGGCGC-UCGUCCag -5' |
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13944 | 3' | -57.1 | NC_003521.1 | + | 90914 | 0.67 | 0.912764 |
Target: 5'- gGCGUC-GGUGUUGuuGCcGGGCAa--- -3' miRNA: 3'- -CGCAGuCCACAACggCG-CUCGUccag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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