Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13951 | 5' | -54.8 | NC_003521.1 | + | 83689 | 0.66 | 0.974967 |
Target: 5'- cGUGAC--GUUGuGGAcgcgcgagCGGGCGUCCAa -3' miRNA: 3'- -CAUUGaaCGAC-CCUa-------GCCCGCAGGUg -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 25652 | 0.66 | 0.97234 |
Target: 5'- --cGCggGuCUGGGAgcaGGGUGUCCGg -3' miRNA: 3'- cauUGaaC-GACCCUag-CCCGCAGGUg -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 137492 | 0.66 | 0.97234 |
Target: 5'- -cGGCcggUGCgcggGGGAUCugaaagaaGGGCGUCC-Cg -3' miRNA: 3'- caUUGa--ACGa---CCCUAG--------CCCGCAGGuG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 92066 | 0.66 | 0.97234 |
Target: 5'- cGUAGCgaugGCggaccgGGGGUCGGGgGgUgCGCa -3' miRNA: 3'- -CAUUGaa--CGa-----CCCUAGCCCgC-AgGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 226395 | 0.66 | 0.96952 |
Target: 5'- ------cGCUGGuGGUgGGGCaUCCGCg -3' miRNA: 3'- cauugaaCGACC-CUAgCCCGcAGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 33467 | 0.66 | 0.96952 |
Target: 5'- cGUGGCccUGaCUGGaGAcccagaucUCGGGCG-CCACg -3' miRNA: 3'- -CAUUGa-AC-GACC-CU--------AGCCCGCaGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 19248 | 0.66 | 0.963275 |
Target: 5'- ------aGCUGGccgcugUGGGCGUCCACc -3' miRNA: 3'- cauugaaCGACCcua---GCCCGCAGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 227238 | 0.67 | 0.956189 |
Target: 5'- uUGGCUcgGCgcgGGGGUcucugCGGGCGgcgCCGCc -3' miRNA: 3'- cAUUGAa-CGa--CCCUA-----GCCCGCa--GGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 181736 | 0.67 | 0.948225 |
Target: 5'- ------aGCUGGGAU-GGGCcGUCUGCg -3' miRNA: 3'- cauugaaCGACCCUAgCCCG-CAGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 196148 | 0.68 | 0.939358 |
Target: 5'- -aGGCUgacGCUGGGGaUGGGCaGUCC-Cg -3' miRNA: 3'- caUUGAa--CGACCCUaGCCCG-CAGGuG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 193278 | 0.68 | 0.934579 |
Target: 5'- -gAGCgggUGCcGGGA-CGGGCGUCgGa -3' miRNA: 3'- caUUGa--ACGaCCCUaGCCCGCAGgUg -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 17166 | 0.68 | 0.934579 |
Target: 5'- gGUGAUUggagGCgcggccgagGGGGUCGaGGCGgCCGCg -3' miRNA: 3'- -CAUUGAa---CGa--------CCCUAGC-CCGCaGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 81321 | 0.68 | 0.934579 |
Target: 5'- -cGACgUGCUGGGGUUGGcgcaguGCGUCaACa -3' miRNA: 3'- caUUGaACGACCCUAGCC------CGCAGgUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 143573 | 0.68 | 0.929568 |
Target: 5'- ------cGCUGGcGGgcaCGGGCGUCUACc -3' miRNA: 3'- cauugaaCGACC-CUa--GCCCGCAGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 45358 | 0.68 | 0.929568 |
Target: 5'- gGUAGCg-GCUGGGGugaUCGGGCacgcggaagauGUCCGg -3' miRNA: 3'- -CAUUGaaCGACCCU---AGCCCG-----------CAGGUg -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 135323 | 0.68 | 0.922163 |
Target: 5'- -cAACagGCcGGGGUCGGGCGgguugccucccucCCGCg -3' miRNA: 3'- caUUGaaCGaCCCUAGCCCGCa------------GGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 216376 | 0.68 | 0.91885 |
Target: 5'- -gAGCagaaggGCUGcc-UCGGGCGUCCACg -3' miRNA: 3'- caUUGaa----CGACccuAGCCCGCAGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 225552 | 0.68 | 0.913142 |
Target: 5'- uGUGGCgaacgcGCUGGGAagaggGGGCGcCCACc -3' miRNA: 3'- -CAUUGaa----CGACCCUag---CCCGCaGGUG- -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 132442 | 0.69 | 0.888029 |
Target: 5'- gGUAGCggUGgUGGGcuguUCGGcGCGUCCGg -3' miRNA: 3'- -CAUUGa-ACgACCCu---AGCC-CGCAGGUg -5' |
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13951 | 5' | -54.8 | NC_003521.1 | + | 53407 | 0.7 | 0.835841 |
Target: 5'- ---cCUUGC-GGGcGUCGGGCG-CCACc -3' miRNA: 3'- cauuGAACGaCCC-UAGCCCGCaGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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