Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13953 | 5' | -44.1 | NC_003521.1 | + | 34120 | 0.66 | 1 |
Target: 5'- --cGUAACGAAagUCGUCGAUgAgCAg -3' miRNA: 3'- uuuCAUUGCUUgaAGCAGCUAgUaGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 63429 | 0.66 | 1 |
Target: 5'- -uAGUcugGGGCggcgCGUCGAUCGUCAg -3' miRNA: 3'- uuUCAuugCUUGaa--GCAGCUAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 170750 | 0.67 | 1 |
Target: 5'- --cGUGACGAuCUUCG-CGAUCGc-- -3' miRNA: 3'- uuuCAUUGCUuGAAGCaGCUAGUagu -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 159857 | 0.66 | 1 |
Target: 5'- --cGUAACGGuuGCUucuccucaUCGUCG-UCAUCGg -3' miRNA: 3'- uuuCAUUGCU--UGA--------AGCAGCuAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 98023 | 0.68 | 1 |
Target: 5'- -uGGUGACGAugU-CGUCGucacCAUCGc -3' miRNA: 3'- uuUCAUUGCUugAaGCAGCua--GUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 3904 | 0.68 | 1 |
Target: 5'- uGAGUGcacccggaACGAugUUUGuUCGAUCGUUAu -3' miRNA: 3'- uUUCAU--------UGCUugAAGC-AGCUAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 234220 | 0.68 | 0.999998 |
Target: 5'- uGGGUAGCGGAUc-CGUCG-UCGUCGu -3' miRNA: 3'- uUUCAUUGCUUGaaGCAGCuAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 105201 | 0.69 | 0.999996 |
Target: 5'- cGAGUAACcGACggCGUCGcgcucGUCGUCAu -3' miRNA: 3'- uUUCAUUGcUUGaaGCAGC-----UAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 142000 | 0.69 | 0.999995 |
Target: 5'- --cGUGAUGAGCccggCGUCGGUCAa-- -3' miRNA: 3'- uuuCAUUGCUUGaa--GCAGCUAGUagu -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 179852 | 0.7 | 0.999972 |
Target: 5'- -uAGUAACGGAUgugaUCGUCG-UCGUCc -3' miRNA: 3'- uuUCAUUGCUUGa---AGCAGCuAGUAGu -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 58164 | 0.7 | 0.999972 |
Target: 5'- -cGGUAGCGGGCcaCGUUGA-CAUCAu -3' miRNA: 3'- uuUCAUUGCUUGaaGCAGCUaGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 239531 | 0.71 | 0.999949 |
Target: 5'- --cGUAGCGGAaccaUUCGUCGG-CGUCGc -3' miRNA: 3'- uuuCAUUGCUUg---AAGCAGCUaGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 201784 | 0.71 | 0.999949 |
Target: 5'- --cGUAGCGGAaccaUUCGUCGG-CGUCGc -3' miRNA: 3'- uuuCAUUGCUUg---AAGCAGCUaGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 110360 | 0.71 | 0.999882 |
Target: 5'- aAGAGcaggAGCGcuGGCgcgUCGUCGAUCGUCGc -3' miRNA: 3'- -UUUCa---UUGC--UUGa--AGCAGCUAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 210917 | 0.72 | 0.999746 |
Target: 5'- ---uUGACGGccACgggUCGUCGGUCGUCGu -3' miRNA: 3'- uuucAUUGCU--UGa--AGCAGCUAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 220152 | 0.73 | 0.999208 |
Target: 5'- gAAGGUGAUGAACagCGccaCGGUCAUCAc -3' miRNA: 3'- -UUUCAUUGCUUGaaGCa--GCUAGUAGU- -5' |
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13953 | 5' | -44.1 | NC_003521.1 | + | 23836 | 1.07 | 0.07943 |
Target: 5'- cAAAGUAACGAACUUCGUCGAUCAUCAc -3' miRNA: 3'- -UUUCAUUGCUUGAAGCAGCUAGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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