Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13954 | 3' | -53.3 | NC_003521.1 | + | 192290 | 0.67 | 0.989374 |
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Target: 5'- aCGCCG-UUCGUCGGcacCGUcCGCgGUa -3' miRNA: 3'- gGCGGCuAAGUAGCCua-GCA-GCGaCA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 16945 | 0.66 | 0.99175 |
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Target: 5'- aCCGCCGuuacCGUCGGGga-UUGCUGg -3' miRNA: 3'- -GGCGGCuaa-GUAGCCUagcAGCGACa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 192681 | 0.66 | 0.992769 |
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Target: 5'- cUCGCCGAUguaCA-CGGAggugcacaggUCGUCGCg-- -3' miRNA: 3'- -GGCGGCUAa--GUaGCCU----------AGCAGCGaca -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 86446 | 0.66 | 0.992769 |
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Target: 5'- aCGUCGAUgagGUCGuGAUCGuuguagUCGCUGg -3' miRNA: 3'- gGCGGCUAag-UAGC-CUAGC------AGCGACa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 210920 | 0.66 | 0.993685 |
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Target: 5'- aCgGCCacGggUCGUCGGucGUCGUCGCccagGUg -3' miRNA: 3'- -GgCGG--CuaAGUAGCC--UAGCAGCGa---CA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 239527 | 0.66 | 0.994505 |
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Target: 5'- gCGCCGuagcggaaccAUUCGUCGG--CGUCGCgGg -3' miRNA: 3'- gGCGGC----------UAAGUAGCCuaGCAGCGaCa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 82527 | 0.66 | 0.994505 |
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Target: 5'- cCUGCUGAgUCGUcCGGGUgGUCGUa-- -3' miRNA: 3'- -GGCGGCUaAGUA-GCCUAgCAGCGaca -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 105194 | 0.66 | 0.994505 |
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Target: 5'- cCCGCCGA-UCGUgGGAUCcgcccgcgaGCUGc -3' miRNA: 3'- -GGCGGCUaAGUAgCCUAGcag------CGACa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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