Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13955 | 3' | -49.8 | NC_003521.1 | + | 216284 | 0.66 | 0.999351 |
Target: 5'- gGCC-CUGGGCCcuGCGcuugCUGGUGGGc -3' miRNA: 3'- -CGGaGACUUGGu-CGCaa--GAUCACUUu -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 139080 | 0.66 | 0.999202 |
Target: 5'- gGCCUCggggcUGGACUAcGUGUUCggcGUGAAGg -3' miRNA: 3'- -CGGAG-----ACUUGGU-CGCAAGau-CACUUU- -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 212963 | 0.66 | 0.999185 |
Target: 5'- aGgCUCUGAGgauuuaucuuugcCCAGCGgcucgccCUGGUGAAAc -3' miRNA: 3'- -CgGAGACUU-------------GGUCGCaa-----GAUCACUUU- -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 62039 | 0.66 | 0.999024 |
Target: 5'- cGCCg--GAGCCGGCGgcggUCccGGUGAc- -3' miRNA: 3'- -CGGagaCUUGGUCGCa---AGa-UCACUuu -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 153627 | 0.66 | 0.998814 |
Target: 5'- aGCUcCUGcGCCAGCGggugcgUCUcGGUGGAGc -3' miRNA: 3'- -CGGaGACuUGGUCGCa-----AGA-UCACUUU- -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 101530 | 0.67 | 0.998275 |
Target: 5'- cGCgCUCUGGGCCAGCc-UCUGGa---- -3' miRNA: 3'- -CG-GAGACUUGGUCGcaAGAUCacuuu -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 104059 | 0.67 | 0.997543 |
Target: 5'- cGCCgUCUGcGCCAGCacgUUGGUGAc- -3' miRNA: 3'- -CGG-AGACuUGGUCGcaaGAUCACUuu -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 207175 | 0.67 | 0.997543 |
Target: 5'- gGCCgUCUGAAuCCAGCGggau-GUGAGc -3' miRNA: 3'- -CGG-AGACUU-GGUCGCaagauCACUUu -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 118676 | 0.67 | 0.99709 |
Target: 5'- gGCUUCggGAGgCGGCGguaccgCUGGUGGAGg -3' miRNA: 3'- -CGGAGa-CUUgGUCGCaa----GAUCACUUU- -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 104751 | 0.7 | 0.98593 |
Target: 5'- gGCCUCUGucgcGGCCAGCcacUUGGUGggGg -3' miRNA: 3'- -CGGAGAC----UUGGUCGcaaGAUCACuuU- -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 46113 | 0.7 | 0.977696 |
Target: 5'- cGCCUCcGGACgCAGCGUUCgcagGGAGg -3' miRNA: 3'- -CGGAGaCUUG-GUCGCAAGaucaCUUU- -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 231410 | 0.74 | 0.8902 |
Target: 5'- gGCCgUCUucGCCAGCGUcuUCUGGUGGc- -3' miRNA: 3'- -CGG-AGAcuUGGUCGCA--AGAUCACUuu -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 25294 | 0.77 | 0.772457 |
Target: 5'- gGCCUgUGuAACUAGCcUUCUAGUGAAAg -3' miRNA: 3'- -CGGAgAC-UUGGUCGcAAGAUCACUUU- -5' |
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13955 | 3' | -49.8 | NC_003521.1 | + | 25603 | 1.09 | 0.015587 |
Target: 5'- aGCCUCUGAACCAGCGUUCUAGUGAAAu -3' miRNA: 3'- -CGGAGACUUGGUCGCAAGAUCACUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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