Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13955 | 5' | -45.1 | NC_003521.1 | + | 46677 | 0.66 | 1 |
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Target: 5'- cCUcgCACgAGGAGGCcgAGggACGCAu -3' miRNA: 3'- uGAaaGUGaUCUUCCGa-UCaaUGUGU- -5' |
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| 13955 | 5' | -45.1 | NC_003521.1 | + | 204251 | 0.67 | 0.999999 |
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Target: 5'- gGCcagUUCGCgcaugGGGAGGCUGGagacgGCGCu -3' miRNA: 3'- -UGa--AAGUGa----UCUUCCGAUCaa---UGUGu -5' |
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| 13955 | 5' | -45.1 | NC_003521.1 | + | 204256 | 0.67 | 0.999999 |
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Target: 5'- --gUUCGCgcaugGGGAGGCUGGagacgGCGCu -3' miRNA: 3'- ugaAAGUGa----UCUUCCGAUCaa---UGUGu -5' |
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| 13955 | 5' | -45.1 | NC_003521.1 | + | 55993 | 0.67 | 0.999997 |
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Target: 5'- cGCg--CGCUGGGAGGCgc--UGCGCGc -3' miRNA: 3'- -UGaaaGUGAUCUUCCGaucaAUGUGU- -5' |
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| 13955 | 5' | -45.1 | NC_003521.1 | + | 25333 | 0.68 | 0.999988 |
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Target: 5'- cGCaUUUCACUAGAAcGCUGGUU-CAgAg -3' miRNA: 3'- -UG-AAAGUGAUCUUcCGAUCAAuGUgU- -5' |
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| 13955 | 5' | -45.1 | NC_003521.1 | + | 156822 | 0.69 | 0.999957 |
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Target: 5'- gGCggUCGCUGGGcAGGCUGagcacGUUGuCACAg -3' miRNA: 3'- -UGaaAGUGAUCU-UCCGAU-----CAAU-GUGU- -5' |
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| 13955 | 5' | -45.1 | NC_003521.1 | + | 235374 | 0.7 | 0.999719 |
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Target: 5'- gGCUgucuaUCGCUAGggGGUggUAGUguccGCGCGa -3' miRNA: 3'- -UGAa----AGUGAUCuuCCG--AUCAa---UGUGU- -5' |
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| 13955 | 5' | -45.1 | NC_003521.1 | + | 25564 | 1.1 | 0.038979 |
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Target: 5'- aACUUUCACUAGAAGGCUAGUUACACAg -3' miRNA: 3'- -UGAAAGUGAUCUUCCGAUCAAUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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