Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13958 | 5' | -51 | NC_003521.1 | + | 148794 | 0.66 | 0.998417 |
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Target: 5'- aGGAGccGCGGCUGCGgacGUUCaucgUGCgcgGCGa -3' miRNA: 3'- -CUUC--UGCCGAUGCa--CAAG----ACGa--UGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 150660 | 0.68 | 0.991217 |
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Target: 5'- cGAAGGCGGCUcuccggcgGCGgcgccgCUGCUGCu- -3' miRNA: 3'- -CUUCUGCCGA--------UGCacaa--GACGAUGca -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 174031 | 0.69 | 0.985474 |
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Target: 5'- aGGAGACGGCgguggcccgcACGUGg---GCUGCGa -3' miRNA: 3'- -CUUCUGCCGa---------UGCACaagaCGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 191684 | 0.68 | 0.993319 |
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Target: 5'- cGAGGACGGCgaggcgucUGCGUGUUaaacgGCUguacGCGg -3' miRNA: 3'- -CUUCUGCCG--------AUGCACAAga---CGA----UGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 195974 | 0.71 | 0.951252 |
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Target: 5'- uGGAGGCGuuuuacgacacGCUGCGgGcgCUGCUACGUa -3' miRNA: 3'- -CUUCUGC-----------CGAUGCaCaaGACGAUGCA- -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 201758 | 0.67 | 0.995632 |
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Target: 5'- -cGGGCGGCUagggucaGCGUGUUgcGCUGCa- -3' miRNA: 3'- cuUCUGCCGA-------UGCACAAgaCGAUGca -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 213841 | 0.68 | 0.991217 |
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Target: 5'- --cGGCuGCUACGUGgcgCUGCUGuCGg -3' miRNA: 3'- cuuCUGcCGAUGCACaa-GACGAU-GCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 229317 | 0.68 | 0.988624 |
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Target: 5'- uGggGACGGCU-Ca---UCUGCUGCGa -3' miRNA: 3'- -CuuCUGCCGAuGcacaAGACGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 239557 | 0.67 | 0.995632 |
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Target: 5'- -cGGGCGGCUagggucaGCGUGUUgcGCUGCa- -3' miRNA: 3'- cuUCUGCCGA-------UGCACAAgaCGAUGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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