miRNA: 3'- -CUCCACCUGCUC--------CUCaaguUCGGgCU- -5'
miRNA: 3'- -CUCCA---CCUGCUCCUCaa------------GU---------------UCGGGCU- -5'
miRNA: 3'- -CUCCa-CC----------UGCUCCUCA---AGUUCGGGcu -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13959 | 5' | -55.8 | NC_003521.1 | + | 221150 | 0.66 | 0.960735 |
|
Target: 5'- gGAGGUGGGCGAGaucgugguGAGgu--GGCCgGGc -3' miRNA: 3'- -CUCCACCUGCUC--------CUCaaguUCGGgCU- -5' |
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| 13959 | 5' | -55.8 | NC_003521.1 | + | 224187 | 0.67 | 0.923656 |
|
Target: 5'- gGAGGUggaGGACGAGGAGgcggacgaugaagaCGaggaagagccuauggAGCCCGAc -3' miRNA: 3'- -CUCCA---CCUGCUCCUCaa------------GU---------------UCGGGCU- -5' |
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| 13959 | 5' | -55.8 | NC_003521.1 | + | 229317 | 0.67 | 0.93623 |
|
Target: 5'- uGGGGacGGcucaucugcuGCGAGGAGUcccUCGAGCCCc- -3' miRNA: 3'- -CUCCa-CC----------UGCUCCUCA---AGUUCGGGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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