Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1396 | 3' | -55.2 | NC_001335.1 | + | 26381 | 0.66 | 0.669717 |
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Target: 5'- --cUCGGGuCGGaCUCGCAaGgUCUACu -3' miRNA: 3'- ucaAGUCCuGCC-GAGCGU-CgAGAUGc -5' |
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| 1396 | 3' | -55.2 | NC_001335.1 | + | 47507 | 0.67 | 0.657556 |
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Target: 5'- ---gCGGGaguacucGCGGUUCGCGGC-CUGCa -3' miRNA: 3'- ucaaGUCC-------UGCCGAGCGUCGaGAUGc -5' |
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| 1396 | 3' | -55.2 | NC_001335.1 | + | 34144 | 0.67 | 0.613195 |
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Target: 5'- gAGUUCuggcgucuggccaAGGAgGGCUacgGCAGCUUcGCGg -3' miRNA: 3'- -UCAAG-------------UCCUgCCGAg--CGUCGAGaUGC- -5' |
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| 1396 | 3' | -55.2 | NC_001335.1 | + | 19504 | 0.68 | 0.565815 |
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Target: 5'- cGcgCAGG-CGGaagucgccaucgCGCAGCUCUACGu -3' miRNA: 3'- uCaaGUCCuGCCga----------GCGUCGAGAUGC- -5' |
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| 1396 | 3' | -55.2 | NC_001335.1 | + | 38668 | 0.71 | 0.396877 |
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Target: 5'- cGUUCAGGACGGCggcgaagcccUCGcCGGUcuccgCUGCGu -3' miRNA: 3'- uCAAGUCCUGCCG----------AGC-GUCGa----GAUGC- -5' |
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| 1396 | 3' | -55.2 | NC_001335.1 | + | 39154 | 1.09 | 0.000844 |
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Target: 5'- gAGUUCAGGACGGCUCGCAGCUCUACGa -3' miRNA: 3'- -UCAAGUCCUGCCGAGCGUCGAGAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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