Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13961 | 3' | -53 | NC_003521.1 | + | 98490 | 0.68 | 0.972668 |
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Target: 5'- ---gUCGUGGgcaacgAGGGCuGGgUGGUCGUCg -3' miRNA: 3'- caugAGUACU------UCUCG-CCgACCAGCAG- -5' |
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| 13961 | 3' | -53 | NC_003521.1 | + | 121418 | 0.67 | 0.975305 |
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Target: 5'- gGUGCUugcgCAUGAAGGGCuGCgGGUugcgCGUCa -3' miRNA: 3'- -CAUGA----GUACUUCUCGcCGaCCA----GCAG- -5' |
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| 13961 | 3' | -53 | NC_003521.1 | + | 127238 | 0.67 | 0.982101 |
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Target: 5'- gGUcCUCcacGAAGAGCGGCUcGUCGg- -3' miRNA: 3'- -CAuGAGua-CUUCUCGCCGAcCAGCag -5' |
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| 13961 | 3' | -53 | NC_003521.1 | + | 69812 | 0.67 | 0.984742 |
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Target: 5'- -cGCUCGcacuUGAggcgacacaggcaccAGAGCGGCaGGUCGa- -3' miRNA: 3'- caUGAGU----ACU---------------UCUCGCCGaCCAGCag -5' |
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| 13961 | 3' | -53 | NC_003521.1 | + | 166265 | 0.66 | 0.985779 |
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Target: 5'- cGUGCUCAcGGgcacgcgcacGGcGCGGCUGGUCc-- -3' miRNA: 3'- -CAUGAGUaCU----------UCuCGCCGACCAGcag -5' |
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| 13961 | 3' | -53 | NC_003521.1 | + | 19903 | 0.77 | 0.592102 |
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Target: 5'- -aACggCGUGcGGGAGCGGCUGGUgGUCc -3' miRNA: 3'- caUGa-GUAC-UUCUCGCCGACCAgCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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