Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13961 | 3' | -53 | NC_003521.1 | + | 211806 | 0.7 | 0.931507 |
Target: 5'- cUGCUCuccGGAGAGCaGCuugagcgcgcccuggUGGUCGUCg -3' miRNA: 3'- cAUGAGua-CUUCUCGcCG---------------ACCAGCAG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 54044 | 0.72 | 0.850336 |
Target: 5'- ----aCAUGuGGAcuGCGGUUGGUCGUCg -3' miRNA: 3'- caugaGUACuUCU--CGCCGACCAGCAG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 127382 | 0.75 | 0.704021 |
Target: 5'- gGUGCgUgAUGAAGGGCGGCcacaggcggUGGUCGUg -3' miRNA: 3'- -CAUG-AgUACUUCUCGCCG---------ACCAGCAg -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 19903 | 0.77 | 0.592102 |
Target: 5'- -aACggCGUGcGGGAGCGGCUGGUgGUCc -3' miRNA: 3'- caUGa-GUAC-UUCUCGCCGACCAgCAG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 124470 | 0.77 | 0.58195 |
Target: 5'- -aGCUCGgccucGucGGGCGGCUGGUCGUa -3' miRNA: 3'- caUGAGUa----CuuCUCGCCGACCAGCAg -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 211561 | 0.97 | 0.043577 |
Target: 5'- cGUACUCAUGAAGAGC-GCUGGUCGUCg -3' miRNA: 3'- -CAUGAGUACUUCUCGcCGACCAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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