Results 81 - 83 of 83 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
13964 | 3' | -57.9 | NC_003521.1 | + | 170052 | 0.66 | 0.924144 |
Target: 5'- gCCAUGAGGUUGGcguucccGGUCGCGUu--- -3' miRNA: 3'- gGGUGCUCCAGCCu------CCAGUGCGcugu -5' |
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13964 | 3' | -57.9 | NC_003521.1 | + | 7962 | 0.66 | 0.924144 |
Target: 5'- gCCGCGGugucgcGGcCGGgaGGGUC-CGCGGCGg -3' miRNA: 3'- gGGUGCU------CCaGCC--UCCAGuGCGCUGU- -5' |
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13964 | 3' | -57.9 | NC_003521.1 | + | 132430 | 0.66 | 0.942958 |
Target: 5'- -aCACGGGGUCGGGuuagCGUGCGGCGc -3' miRNA: 3'- ggGUGCUCCAGCCUcca-GUGCGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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