miRNA: 3'- -GGGUGcUCCAGCcUCCaGUGC-GCUGu -5'
miRNA: 3'- -GGGUGCU--CCaGCCUCCag---UGC-GCUGU- -5'
miRNA: 3'- -GGGUGCUCCAGCCUCCAGUGCGCUGU- -5'
Results 81 - 83 of 83 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13964 | 3' | -57.9 | NC_003521.1 | + | 180561 | 0.69 | 0.840273 |
|
Target: 5'- cCCCACcAGGUCGuGGGcCACGuCGAUc -3' miRNA: 3'- -GGGUGcUCCAGCcUCCaGUGC-GCUGu -5' |
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| 13964 | 3' | -57.9 | NC_003521.1 | + | 135639 | 0.69 | 0.840273 |
|
Target: 5'- uCCCACGAucGG-CGGGGGgcccaGCGuCGGCGg -3' miRNA: 3'- -GGGUGCU--CCaGCCUCCag---UGC-GCUGU- -5' |
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| 13964 | 3' | -57.9 | NC_003521.1 | + | 210095 | 1.1 | 0.003486 |
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Target: 5'- cCCCACGAGGUCGGAGGUCACGCGACAu -3' miRNA: 3'- -GGGUGCUCCAGCCUCCAGUGCGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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