Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13964 | 5' | -50.4 | NC_003521.1 | + | 46446 | 0.7 | 0.982762 |
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Target: 5'- cGGgGCUGCGggGgcugGUcGGCCUCGUc -3' miRNA: 3'- -CCgCGGCGCaaCaa--UAuUCGGAGUAc -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 43387 | 0.67 | 0.998289 |
|
Target: 5'- uGGCGCCGCc-UGgccauGGCCUCu-- -3' miRNA: 3'- -CCGCGGCGcaACaauauUCGGAGuac -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 36898 | 0.7 | 0.982761 |
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Target: 5'- aGGCaGCCGUGgUGgaagAGGCUUCGUGu -3' miRNA: 3'- -CCG-CGGCGCaACaauaUUCGGAGUAC- -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 32164 | 0.69 | 0.989374 |
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Target: 5'- aGGCGcCCGCGUUGUUccAUcgaaagugcugcGGGUCgggCAUGg -3' miRNA: 3'- -CCGC-GGCGCAACAA--UA------------UUCGGa--GUAC- -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 30711 | 0.69 | 0.990667 |
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Target: 5'- cGGCGCCGCGggcucaUGUcgcGUGAccuccGaCCUCGUGg -3' miRNA: 3'- -CCGCGGCGCa-----ACAa--UAUU-----C-GGAGUAC- -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 25570 | 0.66 | 0.999199 |
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Target: 5'- aGGCgGCCGCGggGacagGUGGGCUuguUCAUc -3' miRNA: 3'- -CCG-CGGCGCaaCaa--UAUUCGG---AGUAc -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 12204 | 0.66 | 0.998573 |
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Target: 5'- cGCGCCGCGggGUUcu---CCUCAc- -3' miRNA: 3'- cCGCGGCGCaaCAAuauucGGAGUac -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 5705 | 0.67 | 0.997734 |
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Target: 5'- gGGCGCCGUcaccGggGUUAUGuguacGGCCcgggcacggcgcgugUCAUGg -3' miRNA: 3'- -CCGCGGCG----CaaCAAUAU-----UCGG---------------AGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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