Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1397 | 5' | -61.9 | NC_001335.1 | + | 14471 | 0.66 | 0.363388 |
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Target: 5'- aGguCAaGGGCUCCUggcagaagaagaaGCUC-CGCGAGa -3' miRNA: 3'- gCguGUcCCCGAGGG-------------CGAGuGCGCUC- -5' |
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| 1397 | 5' | -61.9 | NC_001335.1 | + | 31870 | 0.67 | 0.343136 |
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Target: 5'- gGCugAccGGGGCUugccgcgcaagcaggCCCGCgaggcaGCGCGAGc -3' miRNA: 3'- gCGugU--CCCCGA---------------GGGCGag----UGCGCUC- -5' |
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| 1397 | 5' | -61.9 | NC_001335.1 | + | 18538 | 0.71 | 0.160864 |
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Target: 5'- aCGCgaaGCAGGGGCcgcgcUCCUgGCUguCGCGAGa -3' miRNA: 3'- -GCG---UGUCCCCG-----AGGG-CGAguGCGCUC- -5' |
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| 1397 | 5' | -61.9 | NC_001335.1 | + | 5471 | 0.72 | 0.156683 |
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Target: 5'- aGUACuucGGGGUugUCCCGCUCGaugucgGCGAGg -3' miRNA: 3'- gCGUGu--CCCCG--AGGGCGAGUg-----CGCUC- -5' |
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| 1397 | 5' | -61.9 | NC_001335.1 | + | 4395 | 0.75 | 0.088968 |
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Target: 5'- --gGCAGGGGCUCCCcacGCUCugGgGAa -3' miRNA: 3'- gcgUGUCCCCGAGGG---CGAGugCgCUc -5' |
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| 1397 | 5' | -61.9 | NC_001335.1 | + | 39222 | 1.08 | 0.000257 |
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Target: 5'- uCGCACAGGGGCUCCCGCUCACGCGAGa -3' miRNA: 3'- -GCGUGUCCCCGAGGGCGAGUGCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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