Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1398 | 5' | -51.5 | NC_001335.1 | + | 5975 | 0.66 | 0.901286 |
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Target: 5'- gCGGCGUUCCCG--AUUGGCcgUCGGu- -3' miRNA: 3'- -GCCGUAAGGGCagUAGUUGa-AGCCuc -5' |
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| 1398 | 5' | -51.5 | NC_001335.1 | + | 24814 | 0.66 | 0.871032 |
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Target: 5'- gCGGCAUacaUCCUGgCGUgGACgagccuccggUUCGGAGa -3' miRNA: 3'- -GCCGUA---AGGGCaGUAgUUG----------AAGCCUC- -5' |
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| 1398 | 5' | -51.5 | NC_001335.1 | + | 39641 | 0.67 | 0.827437 |
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Target: 5'- -aGCGUUCCCaGUCGgcucggugCAGC-UCGGGGa -3' miRNA: 3'- gcCGUAAGGG-CAGUa-------GUUGaAGCCUC- -5' |
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| 1398 | 5' | -51.5 | NC_001335.1 | + | 17749 | 0.68 | 0.798617 |
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Target: 5'- aGGCAggguucaagCCCGUCAUCGAa--CGGGc -3' miRNA: 3'- gCCGUaa-------GGGCAGUAGUUgaaGCCUc -5' |
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| 1398 | 5' | -51.5 | NC_001335.1 | + | 16793 | 0.69 | 0.768152 |
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Target: 5'- uCGGCggUCCgcugucgaCGuUCAUCAACggguUCGGAGa -3' miRNA: 3'- -GCCGuaAGG--------GC-AGUAGUUGa---AGCCUC- -5' |
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| 1398 | 5' | -51.5 | NC_001335.1 | + | 3399 | 0.69 | 0.747086 |
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Target: 5'- aCGGCAccaacacCCCGgcCAUCGACUacaacgacgugUCGGAGa -3' miRNA: 3'- -GCCGUaa-----GGGCa-GUAGUUGA-----------AGCCUC- -5' |
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| 1398 | 5' | -51.5 | NC_001335.1 | + | 21317 | 0.71 | 0.623954 |
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Target: 5'- aCGGCAacaUCCaCGUCAgUCAGCUUCcugaccaGGAGa -3' miRNA: 3'- -GCCGUa--AGG-GCAGU-AGUUGAAG-------CCUC- -5' |
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| 1398 | 5' | -51.5 | NC_001335.1 | + | 39568 | 1.1 | 0.00206 |
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Target: 5'- cCGGCAUUCCCGUCAUCAACUUCGGAGa -3' miRNA: 3'- -GCCGUAAGGGCAGUAGUUGAAGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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