Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13982 | 3' | -49.5 | NC_003521.1 | + | 166881 | 0.66 | 0.999702 |
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Target: 5'- --gGUGCugG-UCUGGU---UCUUGGu -3' miRNA: 3'- cuaCACGugCaAGACCGucaAGAACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 26287 | 0.67 | 0.999288 |
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Target: 5'- --gGUGCugGUgCUgGGCA--UCUUGGa -3' miRNA: 3'- cuaCACGugCAaGA-CCGUcaAGAACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 105294 | 0.67 | 0.998939 |
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Target: 5'- ----aGCGCGUUCaUGGCGGgcg-UGGg -3' miRNA: 3'- cuacaCGUGCAAG-ACCGUCaagaACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 140092 | 0.67 | 0.998715 |
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Target: 5'- cGggGUGCAUcaUCUGGUAGcgCUUGc -3' miRNA: 3'- -CuaCACGUGcaAGACCGUCaaGAACc -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 165842 | 0.67 | 0.998715 |
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Target: 5'- aGAUGUccagGCAgGUgugCUGGCGGUgCgagUGGa -3' miRNA: 3'- -CUACA----CGUgCAa--GACCGUCAaGa--ACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 178628 | 0.69 | 0.995037 |
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Target: 5'- gGAUuUGCACGUUgcgucgUUGGCGGUcgaugccgUCUUGGc -3' miRNA: 3'- -CUAcACGUGCAA------GACCGUCA--------AGAACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 77740 | 0.71 | 0.981606 |
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Target: 5'- --gGUGgGCGgcgUCUGGUAGUggUUGGg -3' miRNA: 3'- cuaCACgUGCa--AGACCGUCAagAACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 86528 | 0.74 | 0.915199 |
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Target: 5'- cGAUGgGCACGUUCUugGGCAGagCgUUGGa -3' miRNA: 3'- -CUACaCGUGCAAGA--CCGUCaaG-AACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 101217 | 0.82 | 0.547835 |
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Target: 5'- aGAUGUGCACGUUUuuggcguuggagUGGCAcUUCUUGGu -3' miRNA: 3'- -CUACACGUGCAAG------------ACCGUcAAGAACC- -5' |
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| 13982 | 3' | -49.5 | NC_003521.1 | + | 204163 | 1.11 | 0.013288 |
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Target: 5'- cGAUGUGCACGUUCUGGCAGUUCUUGGu -3' miRNA: 3'- -CUACACGUGCAAGACCGUCAAGAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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