Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13997 | 5' | -51.8 | NC_003521.1 | + | 173031 | 0.66 | 0.995355 |
Target: 5'- aGCACGCgcucgCCggugggcagcugggUCUGGGUGUUggcuucgUUCAGCa -3' miRNA: 3'- -CGUGCGa----GGa-------------AGGUCCACAG-------AAGUUG- -5' |
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13997 | 5' | -51.8 | NC_003521.1 | + | 209713 | 0.66 | 0.99695 |
Target: 5'- -gGCGCUCCaggUCCgAGGagGUCgugcgCAGCg -3' miRNA: 3'- cgUGCGAGGa--AGG-UCCa-CAGaa---GUUG- -5' |
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13997 | 5' | -51.8 | NC_003521.1 | + | 224869 | 0.66 | 0.994375 |
Target: 5'- aGCGCGCcagCUcgUCCAGGUG-CUUggcgcaCAGCa -3' miRNA: 3'- -CGUGCGa--GGa-AGGUCCACaGAA------GUUG- -5' |
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13997 | 5' | -51.8 | NC_003521.1 | + | 89088 | 0.66 | 0.997142 |
Target: 5'- aCGCGCUUCUUcuuucgcaucacccgCCAGGacgaggaccacGUCUUCAGCc -3' miRNA: 3'- cGUGCGAGGAA---------------GGUCCa----------CAGAAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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