miRNA: 3'- -CGUGCGAGGA--------AGGuCCACAGAa------GUUG- -5'
miRNA: 3'- -CGUGCGAGGAa--GGUCca-CAGAAGU--UG- -5'
miRNA: 3'- cgUGCG--AGGAAGGUCCACAGAaGUUG- -5'
miRNA: 3'- -CGUGCGAGGAAGGUCCACAGAAGUUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13997 | 5' | -51.8 | NC_003521.1 | + | 32588 | 0.71 | 0.934509 |
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Target: 5'- aGCAUGCUCCUgcuguggcUCU-GGUGUCUguaccugCGACu -3' miRNA: 3'- -CGUGCGAGGA--------AGGuCCACAGAa------GUUG- -5' |
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| 13997 | 5' | -51.8 | NC_003521.1 | + | 43022 | 0.72 | 0.893749 |
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Target: 5'- uGCACGCUCUUcaaCCAGcugGUCUUCAcgGCa -3' miRNA: 3'- -CGUGCGAGGAa--GGUCca-CAGAAGU--UG- -5' |
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| 13997 | 5' | -51.8 | NC_003521.1 | + | 47315 | 0.79 | 0.559825 |
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Target: 5'- -aACGUccUCUUUCCAGGUGUCUcCAACg -3' miRNA: 3'- cgUGCG--AGGAAGGUCCACAGAaGUUG- -5' |
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| 13997 | 5' | -51.8 | NC_003521.1 | + | 193539 | 1.13 | 0.006353 |
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Target: 5'- gGCACGCUCCUUCCAGGUGUCUUCAACg -3' miRNA: 3'- -CGUGCGAGGAAGGUCCACAGAAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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