Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14004 | 5' | -52.6 | NC_003521.1 | + | 171293 | 0.67 | 0.991184 |
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Target: 5'- aUCCGCGUGggcuggaaggCGGuGCUGGGCCu--- -3' miRNA: 3'- cAGGUGCAUa---------GCCcUGACCUGGuuuc -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 176067 | 0.68 | 0.981669 |
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Target: 5'- aGUCCGCGUGUCagucucuGGGAgUUGGAaaGGGGg -3' miRNA: 3'- -CAGGUGCAUAG-------CCCU-GACCUggUUUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 189648 | 1.08 | 0.01096 |
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Target: 5'- cGUCCACGUAUCGGGACUGGACCAAAGc -3' miRNA: 3'- -CAGGUGCAUAGCCCUGACCUGGUUUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 201348 | 0.67 | 0.988638 |
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Target: 5'- cGUCCACGaggCGGcGACgGGACaCGAAc -3' miRNA: 3'- -CAGGUGCauaGCC-CUGaCCUG-GUUUc -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 201394 | 0.66 | 0.994771 |
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Target: 5'- -gCCACGgcgccgccuaCGGGACUGGACg---- -3' miRNA: 3'- caGGUGCaua-------GCCCUGACCUGguuuc -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 232433 | 0.75 | 0.764659 |
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Target: 5'- -cCUGCGUGUCGGaggucGACUGGACCAc-- -3' miRNA: 3'- caGGUGCAUAGCC-----CUGACCUGGUuuc -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 238692 | 0.69 | 0.969623 |
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Target: 5'- gGUCgGCGgccgUGGGACguuGACCAGAGg -3' miRNA: 3'- -CAGgUGCaua-GCCCUGac-CUGGUUUC- -5' |
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| 14004 | 5' | -52.6 | NC_003521.1 | + | 239254 | 0.66 | 0.995617 |
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Target: 5'- cGUCagcaGCGacggCGGGugUGGcGCCGAGGc -3' miRNA: 3'- -CAGg---UGCaua-GCCCugACC-UGGUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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