Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1402 | 5' | -57.4 | NC_001335.1 | + | 39798 | 0.66 | 0.584099 |
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Target: 5'- -cGgcgGCgCUCgCGGCGCUGACGacaUCGCAg -3' miRNA: 3'- caCa--UG-GAG-GCUGCGGCUGC---AGCGUa -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 8642 | 0.66 | 0.584099 |
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Target: 5'- ---cGCCUCggCGAUGCCGACGagGCu- -3' miRNA: 3'- cacaUGGAG--GCUGCGGCUGCagCGua -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 49256 | 0.66 | 0.551848 |
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Target: 5'- ---aGCCUuuGAcCGCUGGCGUUGUAg -3' miRNA: 3'- cacaUGGAggCU-GCGGCUGCAGCGUa -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 21895 | 0.67 | 0.520157 |
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Target: 5'- ---gGCCaUCGGCGCUGACGgCGCAUu -3' miRNA: 3'- cacaUGGaGGCUGCGGCUGCaGCGUA- -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 14487 | 0.68 | 0.439656 |
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Target: 5'- -cGUGCCUCggcuggUGugGCCGACGcUCGUc- -3' miRNA: 3'- caCAUGGAG------GCugCGGCUGC-AGCGua -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 44945 | 0.7 | 0.349853 |
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Target: 5'- uGUGUuCC-CCGACGCCGugGcCGUc- -3' miRNA: 3'- -CACAuGGaGGCUGCGGCugCaGCGua -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 19273 | 0.71 | 0.287241 |
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Target: 5'- -aGUuacCCUCCGGCucacgagGCCGugGUCGCGc -3' miRNA: 3'- caCAu--GGAGGCUG-------CGGCugCAGCGUa -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 1237 | 0.72 | 0.273894 |
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Target: 5'- ---cGCCUCCGACGCCGccuACGcCGCc- -3' miRNA: 3'- cacaUGGAGGCUGCGGC---UGCaGCGua -5' |
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| 1402 | 5' | -57.4 | NC_001335.1 | + | 40708 | 1.05 | 0.001109 |
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Target: 5'- cGUGUACCUCCGACGCCGACGUCGCAUc -3' miRNA: 3'- -CACAUGGAGGCUGCGGCUGCAGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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