Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1405 | 5' | -51.7 | NC_001335.1 | + | 30313 | 0.66 | 0.88836 |
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Target: 5'- gUGGUCUCagugUCGaCGGCGAGGcaguUCagGUGAg -3' miRNA: 3'- -ACCAGAG----AGC-GCUGCUCU----AGa-UACUg -5' |
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| 1405 | 5' | -51.7 | NC_001335.1 | + | 29157 | 0.67 | 0.809259 |
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Target: 5'- aGG-CUCUCGCugaggcaGugGGGAUCUcgaucccuGUGGCc -3' miRNA: 3'- aCCaGAGAGCG-------CugCUCUAGA--------UACUG- -5' |
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| 1405 | 5' | -51.7 | NC_001335.1 | + | 27039 | 0.68 | 0.769939 |
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Target: 5'- gGGUCUCUccuaCGUGACGAugGGUCUcGUaGGCg -3' miRNA: 3'- aCCAGAGA----GCGCUGCU--CUAGA-UA-CUG- -5' |
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| 1405 | 5' | -51.7 | NC_001335.1 | + | 16050 | 0.7 | 0.670581 |
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Target: 5'- aGGUCUcCUCgGCGGCagccgggggcucgGAGAUCUAggcgaUGACg -3' miRNA: 3'- aCCAGA-GAG-CGCUG-------------CUCUAGAU-----ACUG- -5' |
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| 1405 | 5' | -51.7 | NC_001335.1 | + | 8170 | 0.7 | 0.637813 |
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Target: 5'- cGGUC-CUUGagGGCGAGGUCUGgcacGACg -3' miRNA: 3'- aCCAGaGAGCg-CUGCUCUAGAUa---CUG- -5' |
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| 1405 | 5' | -51.7 | NC_001335.1 | + | 41658 | 1.09 | 0.00215 |
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Target: 5'- cUGGUCUCUCGCGACGAGAUCUAUGACc -3' miRNA: 3'- -ACCAGAGAGCGCUGCUCUAGAUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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